Complete genome amplification of viral RNA provides a new tool for the generation of modified pestiviruses. We have used our full-genome amplification strategy for generation of amplicons representing complete genomes of classical swine fever virus. The amplicons were cloned directly into a stable single-copy bacterial artificial chromosome (BAC) generating full-length pestivirus DNAs from which infectious RNA transcripts could be also derived. Our strategy allows construction of stable infectious BAC DNAs from a single full-length PCR product.
|Publication status||Published - 2011|
|Event||ASF/CSF workshop - Lipica, Slovenia|
Duration: 1 Jan 2011 → …
|Period||01/01/2011 → …|