Complete Genomes of Classical Swine Fever Virus Cloned into Bacterial Artificial Chromosomes

Thomas Bruun Rasmussen, I. Reimann, Åse Uttenthal, M. Beer

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    Abstract

    Complete genome amplification of viral RNA provides a new tool for the generation of modified pestiviruses. We have used our full-genome amplification strategy for generation of amplicons representing complete genomes of classical swine fever virus. The amplicons were cloned directly into a stable single-copy bacterial artificial chromosome (BAC) generating full-length pestivirus DNAs from which infectious RNA transcripts could be also derived. Our strategy allows construction of stable infectious BAC DNAs from a single full-length PCR product.
    Original languageEnglish
    Publication date2011
    Publication statusPublished - 2011
    EventASF/CSF workshop - Lipica, Slovenia
    Duration: 1 Jan 2011 → …

    Conference

    ConferenceASF/CSF workshop
    CityLipica, Slovenia
    Period01/01/2011 → …

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