Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5

Marcelo Bertalan Quintanilha dos Santos; Rodolpho Albano; Vania de Padua; Luc Rouws; Cristian Rojas; Adriana Hemerly; Katia Teixeira; Stefan Schwab; Jean Araujo; Andre Oliveira; Leonardo Franca; Viviane Magalhaes; Sylvia Alqueres; Alexander Cardoso; Welington Almeida; Marcio Martins Loureiro; Eduardo Nogueira; Daniela Cidade; Denise Oliveira; Tatiana Simao; Jacyara Macedo; Ana Valadao; Marcela Dreschsel; Flavia Freitas; Marcia Vidal; Helma Guedes; Elisete Rodrigues; Carlos Meneses; Paulo Brioso; Luciana Pozzer; Daniel Figueiredo; Helena Montano; Jadier Junior; Goncalo de Souza Filho; Victor Martin Quintana Flores; Beatriz Ferreira; Alan Branco; Paula Gonzalez; Heloisa Guillobel; Melissa Lemos; Luiz Seibel; Jose Macedo; Marcio Alves-Ferreira; Gilberto Sachetto-Martins; Ana Coelho; Eidy Santos; Gilda Amaral; Anna Neves; Ana Beatriz Pacheco; Daniela Carvalho; Leticia Lery; Paulo Bisch; Shaila C. Roessle; Turan Uermenyi; Rael Alessandra Pereira; Rosane Silva; Edson Rondinelli; Wanda von Krueger; Orlando Martins; Baldani Jose Ivo; Paulo Ferreira

doi:10.1186/1471-2164-10-450

Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5

Marcelo Bertalan Quintanilha dos Santos, Rodolpho Albano, Vania de Padua, Luc Rouws, Cristian Rojas, Adriana Hemerly, Katia Teixeira, Stefan Schwab, Jean Araujo, Andre Oliveira, Leonardo Franca, Viviane Magalhaes, Sylvia Alqueres, Alexander Cardoso, Welington Almeida, Marcio Martins Loureiro, Eduardo Nogueira, Daniela Cidade, Denise Oliveira, Tatiana SimaoJacyara Macedo, Ana Valadao, Marcela Dreschsel, Flavia Freitas, Marcia Vidal, Helma Guedes, Elisete Rodrigues, Carlos Meneses, Paulo Brioso, Luciana Pozzer, Daniel Figueiredo, Helena Montano, Jadier Junior, Goncalo de Souza Filho, Victor Martin Quintana Flores, Beatriz Ferreira, Alan Branco, Paula Gonzalez, Heloisa Guillobel, Melissa Lemos, Luiz Seibel, Jose Macedo, Marcio Alves-Ferreira, Gilberto Sachetto-Martins, Ana Coelho, Eidy Santos, Gilda Amaral, Anna Neves, Ana Beatriz Pacheco, Daniela Carvalho, Leticia Lery, Paulo Bisch, Shaila C. Roessle, Turan Uermenyi, Rael Alessandra Pereira, Rosane Silva, Edson Rondinelli, Wanda von Krueger, Orlando Martins, Baldani Jose Ivo, Paulo Ferreira

Universidade Federal do Rio de Janeiro

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Background: Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results: Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion: The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.

Original language	English
Journal	B M C Genomics
Volume	10
Pages (from-to)	450
ISSN	1471-2164
DOIs	https://doi.org/10.1186/1471-2164-10-450
Publication status	Published - 2009
Externally published	Yes

Access to Document

10.1186/1471-2164-10-450

OpenUrl availability

Full text

Cite this

dos Santos, M. B. Q., Albano, R., de Padua, V., Rouws, L., Rojas, C., Hemerly, A., Teixeira, K., Schwab, S., Araujo, J., Oliveira, A., Franca, L., Magalhaes, V., Alqueres, S., Cardoso, A., Almeida, W., Loureiro, M. M., Nogueira, E., Cidade, D., Oliveira, D., ... Ferreira, P. (2009). Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5. B M C Genomics, 10, 450. https://doi.org/10.1186/1471-2164-10-450

@article{f1c167ebda504f88bb7aad30e90d7b29,

title = "Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5",

abstract = "Background: Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results: Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion: The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.",

author = "{dos Santos}, {Marcelo Bertalan Quintanilha} and Rodolpho Albano and {de Padua}, Vania and Luc Rouws and Cristian Rojas and Adriana Hemerly and Katia Teixeira and Stefan Schwab and Jean Araujo and Andre Oliveira and Leonardo Franca and Viviane Magalhaes and Sylvia Alqueres and Alexander Cardoso and Welington Almeida and Loureiro, {Marcio Martins} and Eduardo Nogueira and Daniela Cidade and Denise Oliveira and Tatiana Simao and Jacyara Macedo and Ana Valadao and Marcela Dreschsel and Flavia Freitas and Marcia Vidal and Helma Guedes and Elisete Rodrigues and Carlos Meneses and Paulo Brioso and Luciana Pozzer and Daniel Figueiredo and Helena Montano and Jadier Junior and {de Souza Filho}, Goncalo and {Quintana Flores}, {Victor Martin} and Beatriz Ferreira and Alan Branco and Paula Gonzalez and Heloisa Guillobel and Melissa Lemos and Luiz Seibel and Jose Macedo and Marcio Alves-Ferreira and Gilberto Sachetto-Martins and Ana Coelho and Eidy Santos and Gilda Amaral and Anna Neves and Pacheco, {Ana Beatriz} and Daniela Carvalho and Leticia Lery and Paulo Bisch and Roessle, {Shaila C.} and Turan Uermenyi and Pereira, {Rael Alessandra} and Rosane Silva and Edson Rondinelli and {von Krueger}, Wanda and Orlando Martins and {Jose Ivo}, Baldani and Paulo Ferreira",

year = "2009",

doi = "10.1186/1471-2164-10-450",

language = "English",

volume = "10",

pages = "450",

journal = "BMC Genomics",

issn = "1471-2164",

publisher = "BioMed Central Ltd.",

}

dos Santos, MBQ, Albano, R, de Padua, V, Rouws, L, Rojas, C, Hemerly, A, Teixeira, K, Schwab, S, Araujo, J, Oliveira, A, Franca, L, Magalhaes, V, Alqueres, S, Cardoso, A, Almeida, W, Loureiro, MM, Nogueira, E, Cidade, D, Oliveira, D, Simao, T, Macedo, J, Valadao, A, Dreschsel, M, Freitas, F, Vidal, M, Guedes, H, Rodrigues, E, Meneses, C, Brioso, P, Pozzer, L, Figueiredo, D, Montano, H, Junior, J, de Souza Filho, G, Quintana Flores, VM, Ferreira, B, Branco, A, Gonzalez, P, Guillobel, H, Lemos, M, Seibel, L, Macedo, J, Alves-Ferreira, M, Sachetto-Martins, G, Coelho, A, Santos, E, Amaral, G, Neves, A, Pacheco, AB, Carvalho, D, Lery, L, Bisch, P, Roessle, SC, Uermenyi, T, Pereira, RA, Silva, R, Rondinelli, E, von Krueger, W, Martins, O, Jose Ivo, B & Ferreira, P 2009, 'Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5', B M C Genomics, vol. 10, pp. 450. https://doi.org/10.1186/1471-2164-10-450

TY - JOUR

T1 - Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5

AU - dos Santos, Marcelo Bertalan Quintanilha

AU - Albano, Rodolpho

AU - de Padua, Vania

AU - Rouws, Luc

AU - Rojas, Cristian

AU - Hemerly, Adriana

AU - Teixeira, Katia

AU - Schwab, Stefan

AU - Araujo, Jean

AU - Oliveira, Andre

AU - Franca, Leonardo

AU - Magalhaes, Viviane

AU - Alqueres, Sylvia

AU - Cardoso, Alexander

AU - Almeida, Welington

AU - Loureiro, Marcio Martins

AU - Nogueira, Eduardo

AU - Cidade, Daniela

AU - Oliveira, Denise

AU - Simao, Tatiana

AU - Macedo, Jacyara

AU - Valadao, Ana

AU - Dreschsel, Marcela

AU - Freitas, Flavia

AU - Vidal, Marcia

AU - Guedes, Helma

AU - Rodrigues, Elisete

AU - Meneses, Carlos

AU - Brioso, Paulo

AU - Pozzer, Luciana

AU - Figueiredo, Daniel

AU - Montano, Helena

AU - Junior, Jadier

AU - de Souza Filho, Goncalo

AU - Quintana Flores, Victor Martin

AU - Ferreira, Beatriz

AU - Branco, Alan

AU - Gonzalez, Paula

AU - Guillobel, Heloisa

AU - Lemos, Melissa

AU - Seibel, Luiz

AU - Macedo, Jose

AU - Alves-Ferreira, Marcio

AU - Sachetto-Martins, Gilberto

AU - Coelho, Ana

AU - Santos, Eidy

AU - Amaral, Gilda

AU - Neves, Anna

AU - Pacheco, Ana Beatriz

AU - Carvalho, Daniela

AU - Lery, Leticia

AU - Bisch, Paulo

AU - Roessle, Shaila C.

AU - Uermenyi, Turan

AU - Pereira, Rael Alessandra

AU - Silva, Rosane

AU - Rondinelli, Edson

AU - von Krueger, Wanda

AU - Martins, Orlando

AU - Jose Ivo, Baldani

AU - Ferreira, Paulo

PY - 2009

Y1 - 2009

N2 - Background: Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results: Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion: The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.

AB - Background: Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results: Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion: The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.

U2 - 10.1186/1471-2164-10-450

DO - 10.1186/1471-2164-10-450

M3 - Journal article

C2 - 19775431

SN - 1471-2164

VL - 10

SP - 450

JO - BMC Genomics

JF - BMC Genomics

ER -

Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5

Abstract

Access to Document

OpenUrl availability

Fingerprint

Cite this