Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5

Marcelo Bertalan Quintanilha dos Santos, Rodolpho Albano, Vania de Padua, Luc Rouws, Cristian Rojas, Adriana Hemerly, Katia Teixeira, Stefan Schwab, Jean Araujo, Andre Oliveira, Leonardo Franca, Viviane Magalhaes, Sylvia Alqueres, Alexander Cardoso, Welington Almeida, Marcio Martins Loureiro, Eduardo Nogueira, Daniela Cidade, Denise Oliveira, Tatiana SimaoJacyara Macedo, Ana Valadao, Marcela Dreschsel, Flavia Freitas, Marcia Vidal, Helma Guedes, Elisete Rodrigues, Carlos Meneses, Paulo Brioso, Luciana Pozzer, Daniel Figueiredo, Helena Montano, Jadier Junior, Goncalo de Souza Filho, Victor Martin Quintana Flores, Beatriz Ferreira, Alan Branco, Paula Gonzalez, Heloisa Guillobel, Melissa Lemos, Luiz Seibel, Jose Macedo, Marcio Alves-Ferreira, Gilberto Sachetto-Martins, Ana Coelho, Eidy Santos, Gilda Amaral, Anna Neves, Ana Beatriz Pacheco, Daniela Carvalho, Leticia Lery, Paulo Bisch, Shaila C. Roessle, Turan Uermenyi, Rael Alessandra Pereira, Rosane Silva, Edson Rondinelli, Wanda von Krueger, Orlando Martins, Baldani Jose Ivo, Paulo Ferreira

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Background: Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results: Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion: The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.
Original languageEnglish
JournalB M C Genomics
Volume10
Pages (from-to)450
ISSN1471-2164
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint Dive into the research topics of 'Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5'. Together they form a unique fingerprint.

Cite this