Competitive protein adsorption to polymer surface from human serum

Maria Holmberg, Karin Bagger Stibius Jensen, Niels Bent Larsen, Xiaolin Hou

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Surface modification by "soft" plasma polymerisation to obtain a hydrophilic and non-fouling polymer surface has been validated using radioactive labelling. Adsorption to unmodified and modified polymer surfaces, from both single protein and human serum solutions, has been investigated. By using different radioisotopes, albumin and Immunoglobulin G (IgG) adsorption has been monitored simultaneously during competitive adsorption processes, which to our knowledge has not been reported in the literature before. Results show that albumin and IgG adsorption is dependent on adsorption time and on the presence and concentration of other proteins in bulk solutions during adsorption. Generally, lower albumin and IgG adsorption was observed on the modified and more hydrophilic polymer surfaces, but otherwise the modified and unmodified polymer surfaces showed the same adsorption characteristics.
    Original languageEnglish
    JournalJournal of Materials Science: Materials in Medicine
    Volume19
    Issue number5
    Pages (from-to)2179-2185
    ISSN0957-4530
    DOIs
    Publication statusPublished - 2008

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