Comparison of virulence of different Actinobacillus pleuropneumoniae serotypes and biotypes using an aerosol infection model

Mariann Juul Jacobsen, Jens Peter Nielsen, Ragnhild Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    An aerosol infection model for inoculation of pigs with Actinobacillus pleuropneumoniae is described, With this model the virulence of three A. pleuropneumoniae biotype 1 strains representing serotypes 2, 5b and 6, and one Danish biotype 2 were compared using 13-week-old pigs for inoculation. The pigs were sacrificed 24 h after aerosol exposure and lung lesions were evaluated. In pigs exposed to aerosols of suspensions containing 10(4) CFU/ml of serotypes 2, 5b and 6, a number of 5-10 lesions of haemorrhagic necrotizing pneumonia were induced, For the biotype 2 strain the dose creating similar lesions was 10(9) CFU/ml. Repeated experiments confirmed these results showing similar virulence of serotypes 2, 5b and 6 whereas the biotype 2 strain proved less virulent, The aerosol infection model allowed a comparison of the number of A. pleuropneumoniae CFU/liter air which were necessary to induce lung lesions in susceptible pigs, This indicates that the model will be well suited for virulence studies of A. pleuropneumoniae serotypes in pigs.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume49
    Issue number3-4
    Pages (from-to)159-168
    ISSN0378-1135
    DOIs
    Publication statusPublished - 1996

    Keywords

    • aerosol infection model
    • Actinobacillus pleuropneumoniae
    • biotype 1 and 2
    • virulence

    Fingerprint

    Dive into the research topics of 'Comparison of virulence of different Actinobacillus pleuropneumoniae serotypes and biotypes using an aerosol infection model'. Together they form a unique fingerprint.

    Cite this