Abstract
An aerosol infection model for inoculation of pigs with Actinobacillus pleuropneumoniae is described, With this model the virulence of three A. pleuropneumoniae biotype 1 strains representing serotypes 2, 5b and 6, and one Danish biotype 2 were compared using 13-week-old pigs for inoculation. The pigs were sacrificed 24 h after aerosol exposure and lung lesions were evaluated. In pigs exposed to aerosols of suspensions containing 10(4) CFU/ml of serotypes 2, 5b and 6, a number of 5-10 lesions of haemorrhagic necrotizing pneumonia were induced, For the biotype 2 strain the dose creating similar lesions was 10(9) CFU/ml. Repeated experiments confirmed these results showing similar virulence of serotypes 2, 5b and 6 whereas the biotype 2 strain proved less virulent, The aerosol infection model allowed a comparison of the number of A. pleuropneumoniae CFU/liter air which were necessary to induce lung lesions in susceptible pigs, This indicates that the model will be well suited for virulence studies of A. pleuropneumoniae serotypes in pigs.
| Original language | English |
|---|---|
| Journal | Veterinary Microbiology |
| Volume | 49 |
| Issue number | 3-4 |
| Pages (from-to) | 159-168 |
| ISSN | 0378-1135 |
| DOIs | |
| Publication status | Published - 1996 |
Keywords
- aerosol infection model
- Actinobacillus pleuropneumoniae
- biotype 1 and 2
- virulence
Cite this
Jacobsen, M. J., Nielsen, J. P., & Nielsen, R. (1996). Comparison of virulence of different Actinobacillus pleuropneumoniae serotypes and biotypes using an aerosol infection model. Veterinary Microbiology, 49(3-4), 159-168. https://doi.org/10.1016/0378-1135(95)00184-0