Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars

Research output: Contribution to journalJournal article – Annual report year: 2014Researchpeer-review

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Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance.
Original languageEnglish
JournalResearch in Veterinary Science
Volume97
Issue number2
Pages (from-to)455-457
Number of pages3
ISSN0034-5288
DOIs
Publication statusPublished - 2014
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • C-strain, Vaccine, PCR, CSFV, Classical swine fever, DIVA
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