Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars

F. Widén, H. Everett, S. Blome, J. Fernandez Pinero, Åse Uttenthal, M. Cortey, Tanya von Rosen, M. Tignon, L. Liu

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance.
    Original languageEnglish
    JournalResearch in Veterinary Science
    Volume97
    Issue number2
    Pages (from-to)455-457
    Number of pages3
    ISSN0034-5288
    DOIs
    Publication statusPublished - 2014

    Keywords

    • C-strain
    • Vaccine
    • PCR
    • CSFV
    • Classical swine fever
    • DIVA

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