Comparison of two quantitative PCR techniques for porcine circovirus Type 2 (PCV2) nucleic acid in field samples

Charlotte Kristiane Hjulsager, Llorenc Grau-Roma, M. Sibila, Claes Enøe, Lars Erik Larsen, J. Segales

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

    Abstract

    Porcine circovirus type 2 /PCV2) is considered the essential infectious agent of postweaning multisystemic wasting syndrome (PMWS), a global swine disease of devastating economic and animal welfare impact. Most pigs become infected with PCV2 during their life, but only a proportion of them develop PMWS (1). PMWS is associated with a high PCV2 load, and a general threshold of 10 7 copies of PCV2 per ml serum has been suggested for PMWS diagnosis (2,3). The objective of this study was to compare the performance of two different real-time quantitative polymerase chain reaction (qPCR) assays for PCV2 used routinely in two laboratoires located in Denmark (lab 1) and Spain (lab 2), respectively.
    Original languageEnglish
    Publication date2008
    Publication statusPublished - 2008
    Event20th International Pig Veterinary Society Congress - Durban, South Africa
    Duration: 22 Jun 200826 Jun 2008

    Conference

    Conference20th International Pig Veterinary Society Congress
    CountrySouth Africa
    CityDurban
    Period22/06/200826/06/2008

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