@inproceedings{93015eab8ad34e129a61dea44c5bd1d5,
title = "Comparison of soft tissue handling protocols for identifying optical properties and morphology",
abstract = "Sample handling is an important consideration when aiming for replicating in vivo conditions ex vivo for the sake of validating imaging protocols and identifying biomarkers of disease. We tested five different handling methods: snap frozen in isopentane, directly frozen at -80°C, slowly frozen in a cryobox with and without cryopreservation media, and formalin fixed. The samples were imaged using optical coherence tomography (OCT) for qualitative and quantitative validation based on morphological features and optical properties. All handling methods were compared to fresh tissue samples using OCT-derived optical properties and morphological features. The results indicate a significant difference in the optical attenuation coefficient as well as morphological differences between the five different methods and support the hypothesis that proper sample handling is crucial for obtaining translatable results.",
keywords = "Formalin fixation, Fresh, Frozen, Optical coherence tomography, Soft tissue handling, Tissue optics",
author = "Freja H{\o}ier and Untracht, {Gavrielle R.} and Andersen, {Amanda {\O}ster} and Karina Str{\ae}de and Andreas Kj{\ae}r and Andersen, {Peter E.}",
note = "Publisher Copyright: {\textcopyright} 2024 SPIE.; SPIE Photonics Europe 2024 ; Conference date: 07-04-2024 Through 12-04-2024",
year = "2024",
doi = "10.1117/12.3017638",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Jurgen Popp and Csilla Gergely",
booktitle = "Biomedical Spectroscopy, Microscopy, and Imaging III",
address = "United States",
}