Comparison of Pulsed-Field Gel Electrophoresis, Ribotyping, and Plasmid Profiling for Typing of Vibrio anguillarum Serovar O1

Marianne Skov, Karl Pedersen, Jens Laurits Larsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A total of 75 Vibrio anguillarum serogroup O1 strains were studied with respect to their plasmid contents, ribotypes, and pulsed-field gel electrophoresis (PFGE) patterns. Eight plasmid profiles and six ribotypes were demonstrated, and one profile was dominant by both typing methods. In contrast, PFGE had very high discriminatory power, demonstrating 35 profiles. On the basis of PFGE patterns, a similarity matrix and a dendrogram were constructed. The results indicated that Scandinavian strains and southern European isolates (with some exceptions) belong to two different clonal lineages. A few strains from the United States and United Kingdom deviated considerably from each other and from Scandinavian and southern European strains.
Original languageEnglish
JournalAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume61
Issue number4
Pages (from-to)1540-1545
Number of pages6
ISSN0099-2240
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • ANALYTICAL METHOD
  • BACTERIAL GENETICS
  • DISCRIMINATORY POWER
  • FISH PATHOGEN
  • SIMILARITY MATRIX
  • TYPING METHODS
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio anguillarum
  • Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] Pisces
  • 04500, Mathematical biology and statistical methods
  • 07516, Ecology: environmental biology - Wildlife management: aquatic
  • 10010, Comparative biochemistry
  • 10050, Biochemistry methods - General
  • 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines
  • 10054, Biochemistry methods - Proteins, peptides and amino acids
  • 10060, Biochemistry studies - General
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10504, Biophysics - Methods and techniques
  • 10506, Biophysics - Molecular properties and macromolecules
  • 30000, Bacteriology, general and systematic
  • 30500, Morphology and cytology of bacteria
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 32000, Microbiological apparatus, methods and media
  • 34504, Immunology - Bacterial, viral and fungal
  • 36001, Medical and clinical microbiology - General and methods
  • 36002, Medical and clinical microbiology - Bacteriology
  • Chemical Coordination and Homeostasis
  • Conservation
  • Biochemistry and Molecular Biophysics
  • Cell Biology
  • Genetics
  • Immune System
  • Infection
  • Methods and Techniques
  • Physiology
  • Systematics and Taxonomy
  • Wildlife Management

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