Comparison of Pulsed-Field Gel Electrophoresis, Ribotyping, and Plasmid Profiling for Typing of Vibrio anguillarum Serovar O1

Marianne Skov; Karl Pedersen; Jens Laurits Larsen

Comparison of Pulsed-Field Gel Electrophoresis, Ribotyping, and Plasmid Profiling for Typing of Vibrio anguillarum Serovar O1

Marianne Skov, Karl Pedersen, Jens Laurits Larsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A total of 75 Vibrio anguillarum serogroup O1 strains were studied with respect to their plasmid contents, ribotypes, and pulsed-field gel electrophoresis (PFGE) patterns. Eight plasmid profiles and six ribotypes were demonstrated, and one profile was dominant by both typing methods. In contrast, PFGE had very high discriminatory power, demonstrating 35 profiles. On the basis of PFGE patterns, a similarity matrix and a dendrogram were constructed. The results indicated that Scandinavian strains and southern European isolates (with some exceptions) belong to two different clonal lineages. A few strains from the United States and United Kingdom deviated considerably from each other and from Scandinavian and southern European strains.

Original language	English
Journal	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume	61
Issue number	4
Pages (from-to)	1540-1545
Number of pages	6
ISSN	0099-2240
Publication status	Published - 1995
Externally published	Yes

Keywords

ANALYTICAL METHOD
BACTERIAL GENETICS
DISCRIMINATORY POWER
FISH PATHOGEN
SIMILARITY MATRIX
TYPING METHODS
Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio anguillarum
Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] Pisces
04500, Mathematical biology and statistical methods
07516, Ecology: environmental biology - Wildlife management: aquatic
10010, Comparative biochemistry
10050, Biochemistry methods - General
10052, Biochemistry methods - Nucleic acids, purines and pyrimidines
10054, Biochemistry methods - Proteins, peptides and amino acids
10060, Biochemistry studies - General
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
10064, Biochemistry studies - Proteins, peptides and amino acids
10504, Biophysics - Methods and techniques
10506, Biophysics - Molecular properties and macromolecules
30000, Bacteriology, general and systematic
30500, Morphology and cytology of bacteria
31000, Physiology and biochemistry of bacteria
31500, Genetics of bacteria and viruses
32000, Microbiological apparatus, methods and media
34504, Immunology - Bacterial, viral and fungal
36001, Medical and clinical microbiology - General and methods
36002, Medical and clinical microbiology - Bacteriology
Chemical Coordination and Homeostasis
Conservation
Biochemistry and Molecular Biophysics
Cell Biology
Genetics
Immune System
Infection
Methods and Techniques
Physiology
Systematics and Taxonomy
Wildlife Management

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title = "Comparison of Pulsed-Field Gel Electrophoresis, Ribotyping, and Plasmid Profiling for Typing of Vibrio anguillarum Serovar O1",

abstract = "A total of 75 Vibrio anguillarum serogroup O1 strains were studied with respect to their plasmid contents, ribotypes, and pulsed-field gel electrophoresis (PFGE) patterns. Eight plasmid profiles and six ribotypes were demonstrated, and one profile was dominant by both typing methods. In contrast, PFGE had very high discriminatory power, demonstrating 35 profiles. On the basis of PFGE patterns, a similarity matrix and a dendrogram were constructed. The results indicated that Scandinavian strains and southern European isolates (with some exceptions) belong to two different clonal lineages. A few strains from the United States and United Kingdom deviated considerably from each other and from Scandinavian and southern European strains.",

keywords = "ANALYTICAL METHOD, BACTERIAL GENETICS, DISCRIMINATORY POWER, FISH PATHOGEN, SIMILARITY MATRIX, TYPING METHODS, Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio anguillarum, Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] Pisces, 04500, Mathematical biology and statistical methods, 07516, Ecology: environmental biology - Wildlife management: aquatic, 10010, Comparative biochemistry, 10050, Biochemistry methods - General, 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines, 10054, Biochemistry methods - Proteins, peptides and amino acids, 10060, Biochemistry studies - General, 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines, 10064, Biochemistry studies - Proteins, peptides and amino acids, 10504, Biophysics - Methods and techniques, 10506, Biophysics - Molecular properties and macromolecules, 30000, Bacteriology, general and systematic, 30500, Morphology and cytology of bacteria, 31000, Physiology and biochemistry of bacteria, 31500, Genetics of bacteria and viruses, 32000, Microbiological apparatus, methods and media, 34504, Immunology - Bacterial, viral and fungal, 36001, Medical and clinical microbiology - General and methods, 36002, Medical and clinical microbiology - Bacteriology, Chemical Coordination and Homeostasis, Conservation, Biochemistry and Molecular Biophysics, Cell Biology, Genetics, Immune System, Infection, Methods and Techniques, Physiology, Systematics and Taxonomy, Wildlife Management",

author = "Marianne Skov and Karl Pedersen and Larsen, {Jens Laurits}",

year = "1995",

language = "English",

volume = "61",

pages = "1540--1545",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "4",

}

TY - JOUR

T1 - Comparison of Pulsed-Field Gel Electrophoresis, Ribotyping, and Plasmid Profiling for Typing of Vibrio anguillarum Serovar O1

AU - Skov, Marianne

AU - Pedersen, Karl

AU - Larsen, Jens Laurits

PY - 1995

Y1 - 1995

N2 - A total of 75 Vibrio anguillarum serogroup O1 strains were studied with respect to their plasmid contents, ribotypes, and pulsed-field gel electrophoresis (PFGE) patterns. Eight plasmid profiles and six ribotypes were demonstrated, and one profile was dominant by both typing methods. In contrast, PFGE had very high discriminatory power, demonstrating 35 profiles. On the basis of PFGE patterns, a similarity matrix and a dendrogram were constructed. The results indicated that Scandinavian strains and southern European isolates (with some exceptions) belong to two different clonal lineages. A few strains from the United States and United Kingdom deviated considerably from each other and from Scandinavian and southern European strains.

AB - A total of 75 Vibrio anguillarum serogroup O1 strains were studied with respect to their plasmid contents, ribotypes, and pulsed-field gel electrophoresis (PFGE) patterns. Eight plasmid profiles and six ribotypes were demonstrated, and one profile was dominant by both typing methods. In contrast, PFGE had very high discriminatory power, demonstrating 35 profiles. On the basis of PFGE patterns, a similarity matrix and a dendrogram were constructed. The results indicated that Scandinavian strains and southern European isolates (with some exceptions) belong to two different clonal lineages. A few strains from the United States and United Kingdom deviated considerably from each other and from Scandinavian and southern European strains.

KW - ANALYTICAL METHOD

KW - BACTERIAL GENETICS

KW - DISCRIMINATORY POWER

KW - FISH PATHOGEN

KW - SIMILARITY MATRIX

KW - TYPING METHODS

KW - Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio anguillarum

KW - Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] Pisces

KW - 04500, Mathematical biology and statistical methods

KW - 07516, Ecology: environmental biology - Wildlife management: aquatic

KW - 10010, Comparative biochemistry

KW - 10050, Biochemistry methods - General

KW - 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines

KW - 10054, Biochemistry methods - Proteins, peptides and amino acids

KW - 10060, Biochemistry studies - General

KW - 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines

KW - 10064, Biochemistry studies - Proteins, peptides and amino acids

KW - 10504, Biophysics - Methods and techniques

KW - 10506, Biophysics - Molecular properties and macromolecules

KW - 30000, Bacteriology, general and systematic

KW - 30500, Morphology and cytology of bacteria

KW - 31000, Physiology and biochemistry of bacteria

KW - 31500, Genetics of bacteria and viruses

KW - 32000, Microbiological apparatus, methods and media

KW - 34504, Immunology - Bacterial, viral and fungal

KW - 36001, Medical and clinical microbiology - General and methods

KW - 36002, Medical and clinical microbiology - Bacteriology

KW - Chemical Coordination and Homeostasis

KW - Conservation

KW - Biochemistry and Molecular Biophysics

KW - Cell Biology

KW - Genetics

KW - Immune System

KW - Infection

KW - Methods and Techniques

KW - Physiology

KW - Systematics and Taxonomy

KW - Wildlife Management

M3 - Journal article

VL - 61

SP - 1540

EP - 1545

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 4

ER -