Comparison of Polyacetylene Content in Organically and Conventionally Grown Carrots Using a Fast Ultrasonic Liquid Extraction Method

Malene Søltoft, Morten Rosbjørn Eriksen, Anne Wibe Brændholt Träger, John Nielsen, Kristian Holst Laursen, Søren Husted, Ulrich Halekoh, Pia Knuthsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A rapid and sensitive analytical method for quantification of polyacetylenes in carrot roots was developed. The traditional extraction method (stirring) was compared to a new ultrasonic liquid processor (ULP)-based methodology using high-performance liquid chromatography−ultraviolet (HPLC−UV) and mass spectrometry (MS) for identification and quantification of three polyacetylenes. ULP was superior because a significant reduction in extraction time and improved extraction efficiencies were obtained. After optimization, the ULP method showed good selectivity, precision [relative standard deviations (RSDs) of 2.3−3.6%], and recovery (93% of falcarindiol) of the polyacetylenes. The applicability of the method was documented by comparative analyses of carrots grown organically or conventionally in a 2 year field trial study. The average concentrations of falcarindiol, falcarindiol-3-acetate, and falcarinol in year 1 were 222, 30, and 94 μg of falcarindiol equiv/g of dry weight, respectively, and 3−15% lower in year 2. The concentrations were not significantly influenced by the growth system, but a significant year−year variation was observed for falcarindiol-3-acetate.
Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number13
Pages (from-to)7673-7679
ISSN0021-8561
DOIs
Publication statusPublished - 2010

Keywords

  • HPLC-UV and MS
  • Carrots (Daucus carota)
  • polyacetylenes
  • conventional and organic growth systems
  • method development and validation
  • ultrasonic liquid processor (ULP)

Cite this

Søltoft, M., Eriksen, M. R., Träger, A. W. B., Nielsen, J., Laursen, K. H., Husted, S., Halekoh, U., & Knuthsen, P. (2010). Comparison of Polyacetylene Content in Organically and Conventionally Grown Carrots Using a Fast Ultrasonic Liquid Extraction Method. Journal of Agricultural and Food Chemistry, 58(13), 7673-7679. https://doi.org/10.1021/jf101921v