Comparison of microelectrode sensing configurations for impedimetric cell monitoring

Claudia Caviglia; Arto Heiskanen; Thomas Lars Andresen; Jenny Emnéus; M. Carminati; M. Vergani; G. Ferrari; M. Sampietro

Comparison of microelectrode sensing configurations for impedimetric cell monitoring

Claudia Caviglia
, Arto Heiskanen
, Thomas Lars Andresen
, Jenny Emnéus
, M. Carminati
, M. Vergani
, G. Ferrari
, M. Sampietro

Polytechnic University of Milan

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

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Abstract

A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. For the first time, these widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10μm interdigitated microelectrodes using a versatile custom-made monitoring
platform including a 24-channel miniaturized potentiostat. As expected, characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100kHz vs. 24 kHz).

Original language	English
Title of host publication	Proceedings of the International Workshop on Impedance Spectroscopy
Number of pages	2
Publication date	2012
Publication status	Published - 2012
Event	International Workshop on Impedance Spectroscopy - Chemnitz, Germany Duration: 24 Sept 2012 → 26 Sept 2012 Conference number: 7 http://www.tu-chemnitz.de/etit/messtech/iwis/

Conference

Conference	International Workshop on Impedance Spectroscopy
Number	7
Country/Territory	Germany
City	Chemnitz
Period	24/09/2012 → 26/09/2012
Internet address	http://www.tu-chemnitz.de/etit/messtech/iwis/

Keywords

Impedance spectroscopy
Interdigitated electrodes
Cell tracking

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title = "Comparison of microelectrode sensing configurations for impedimetric cell monitoring",

abstract = "A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. For the first time, these widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10μm interdigitated microelectrodes using a versatile custom-made monitoring platform including a 24-channel miniaturized potentiostat. As expected, characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100kHz vs. 24 kHz).",

keywords = "Impedance spectroscopy, Interdigitated electrodes, Cell tracking",

author = "Claudia Caviglia and Arto Heiskanen and Andresen, \{Thomas Lars\} and Jenny Emn{\'e}us and M. Carminati and M. Vergani and G. Ferrari and M. Sampietro",

year = "2012",

language = "English",

booktitle = "Proceedings of the International Workshop on Impedance Spectroscopy",

note = "International Workshop on Impedance Spectroscopy, IWIS12 ; Conference date: 24-09-2012 Through 26-09-2012",

url = "http://www.tu-chemnitz.de/etit/messtech/iwis/",

}

TY - ABST

T1 - Comparison of microelectrode sensing configurations for impedimetric cell monitoring

AU - Caviglia, Claudia

AU - Heiskanen, Arto

AU - Andresen, Thomas Lars

AU - Emnéus, Jenny

AU - Carminati, M.

AU - Vergani, M.

AU - Ferrari, G.

AU - Sampietro, M.

N1 - Conference code: 7

PY - 2012

Y1 - 2012

N2 - A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. For the first time, these widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10μm interdigitated microelectrodes using a versatile custom-made monitoring platform including a 24-channel miniaturized potentiostat. As expected, characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100kHz vs. 24 kHz).

AB - A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. For the first time, these widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10μm interdigitated microelectrodes using a versatile custom-made monitoring platform including a 24-channel miniaturized potentiostat. As expected, characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100kHz vs. 24 kHz).

KW - Impedance spectroscopy

KW - Interdigitated electrodes

KW - Cell tracking

M3 - Conference abstract in proceedings

BT - Proceedings of the International Workshop on Impedance Spectroscopy

T2 - International Workshop on Impedance Spectroscopy

Y2 - 24 September 2012 through 26 September 2012

ER -

Comparison of microelectrode sensing configurations for impedimetric cell monitoring

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Keywords

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