Comparison of microelectrode sensing configurations for impedimetric cell monitoring

Claudia Caviglia, Arto Heiskanen, Thomas Lars Andresen, Jenny Emnéus, M. Carminati, M. Vergani, G. Ferrari, M. Sampietro

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    Abstract

    A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. For the first time, these widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10μm interdigitated microelectrodes using a versatile custom-made monitoring
    platform including a 24-channel miniaturized potentiostat. As expected, characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100kHz vs. 24 kHz).
    Original languageEnglish
    Title of host publicationProceedings of the International Workshop on Impedance Spectroscopy
    Number of pages2
    Publication date2012
    Publication statusPublished - 2012
    EventInternational Workshop on Impedance Spectroscopy - Chemnitz, Germany
    Duration: 24 Sep 201226 Sep 2012
    Conference number: 7
    http://www.tu-chemnitz.de/etit/messtech/iwis/

    Conference

    ConferenceInternational Workshop on Impedance Spectroscopy
    Number7
    CountryGermany
    CityChemnitz
    Period24/09/201226/09/2012
    Internet address

    Keywords

    • Impedance spectroscopy
    • Interdigitated electrodes
    • Cell tracking

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