Abstract
A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. For the first time, these widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10μm interdigitated microelectrodes using a versatile custom-made monitoring
platform including a 24-channel miniaturized potentiostat. As expected, characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100kHz vs. 24 kHz).
platform including a 24-channel miniaturized potentiostat. As expected, characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100kHz vs. 24 kHz).
Original language | English |
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Title of host publication | Proceedings of the International Workshop on Impedance Spectroscopy |
Number of pages | 2 |
Publication date | 2012 |
Publication status | Published - 2012 |
Event | International Workshop on Impedance Spectroscopy - Chemnitz, Germany Duration: 24 Sept 2012 → 26 Sept 2012 Conference number: 7 http://www.tu-chemnitz.de/etit/messtech/iwis/ |
Conference
Conference | International Workshop on Impedance Spectroscopy |
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Number | 7 |
Country/Territory | Germany |
City | Chemnitz |
Period | 24/09/2012 → 26/09/2012 |
Internet address |
Keywords
- Impedance spectroscopy
- Interdigitated electrodes
- Cell tracking