Comparison of different concentration methods for the detection of hepatitis A virus and calicivirus from bottled natural mineral waters

S. Di Pasquale, M Paniconi, B Auricchio, L Orefice, Anna Charlotte Schultz, D De Medici

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

To evaluate the efficiency of different recovery methods of viral RNA from bottled water, a comparison was made of 2 positively and 2 negatively charged membranes that were used for absorbing and releasing HAV virus particles during the filtration of viral spiked bottled water. All the 4 membranes, regardless of charge and pore size, had low level viral recovery. The results show that a considerable number of the virus particles passed through the pores of the membranes instead of being trapped by the electrostatic charges. Two different procedures were then compared using 1.5 L polyethylene bottles spiked with 10-fold serial dilutions of HAV and FCV. The first procedure included an ultrafiltration-based method followed by MiniMag RNA extraction, and the second an ultracentrifugation-based method followed by RNA extraction using QIAamp® viral RNA mini kit. The ultracentrifugation-based method resulted in a better recovery of HAV and FCV when compared to the ultrafiltration-based method.
Original languageEnglish
JournalJournal of Virological Methods
Volume165
Issue number1
Pages (from-to)57-63
ISSN0166-0934
DOIs
Publication statusPublished - 2010

Keywords

  • Ultrafiltration
  • Concentration methods
  • Bottled water
  • Calicivirus
  • Hepatitis A
  • Ultracentrifugation
  • Real Time-PCR

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