Comparison of a commercial biochemical kit and an oligonucleotide probe for identification of environmental isolates of Vibrio vulnificus

A. Dalsgaard, Inger Dalsgaard, L. Høi, J.L. Larsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Methods for the identification and isolation of environmental isolates of Vibrio vulnificus were evaluated. Alkaline peptone water supplemented with polymyxin B and colistin-polymyxin B- cellobiose agar were employed for the isolation of suspected V. vulnificus from water, sediment and shellfish samples. When comparing the identification of putative V. vulnificus obtained with the API 20E assay and an oligonucleotide probe, 29 API 20E profiles were obtained with only four profiles (representing 20 isolates) reaching the identification threshold of V. vulnificus among a total of 66 isolates hybridizing with the probe. The results indicated that, compared with colony hybridization, the API 20E assay was not adequate for the identification of environmental isolates of V. vulnificus.
Original languageEnglish
JournalLetters in Applied Microbiology
Volume22
Issue number2
Pages (from-to)184-188
ISSN0266-8254
Publication statusPublished - 1996

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