Comparison of 2D SURE and 3D CT imaging of cortical vessels in a rat kidney

SUper-Resolution ultrasound imaging using the Erythrocytes (SURE) can visualize blood vessels at about 25 to 50 µm resolution, but validation is required to assess how accurately the vasculature and its morphology are represented. Previous work compared ultrasound imaging to maximum intensity projections (MIP) of micro-CT volumes at an insufficient voxel size of 22.6 µm. Here, a 5 µm voxel size micro-CT volume of a cortical region in an excised rat kidney was acquired to test the hypothesis that blood vessels down to 50 µm in diameter are detected with SURE imaging. For the micro-CT volume, the blood vessels were segmented by an intensity threshold, and local thickness estimates were computed using expanding spheres within the segmentation mask. An affine registration between the SURE image and the micro-CT volume was manually defined, and a MIP of the microCT volume across 2 mm from the ultrasound imaging plane was computed for vessels with diameter estimates greater than 50 µm. The SURE image depicts 12 cortical radial vessels with high intensities and 9 dimmer cortical radial vessels. Notably, the 12 high intensity vessels are also depicted in the microCT projection, but 5 cortical radial vessels and 2 arcuate blood vessels are only visible in the SURE image. Of the faintly depicted cortical radial vessels, 5 vessels are not readily matched to vessels in the micro-CT projection. On the other hand, the microCT projection contains 6 cortical radial vessel segments and 2 arcuate blood vessels not depicted in the SURE image. These discrepancies in the comparison may arise from the challenging registration of the ultrasound focus beam and the micro-CT volume that is complicated further by the different states of the imaged tissue. Despite these challenges, SURE depicts most vessels at least down to 50 µm and even resolves parallel vessels not visible in the micro-CT projection, thus highlighting its clinical potential.