Comparing temporally-focused GPC and CGH for two-photon excitation and optogenetics in turbid media

Mark Jayson Villangca, Andrew Rafael Bañas, Thomas Aabo, Darwin Palima, Jesper Glückstad

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Abstract

Inherent inhomogeneity in turbid media not only hinders imaging but also projection of arbitrary light patterns for excitation or optical manipulation. In this work we compare two of the most popular phase modulation-based techniques in beam shaping. The Generalized Phase Contrast (GPC) method uses a 4f setup that directly converts phase information to intensity. The GPC method has been used with temporal focusing for excitation in two-photon optogenetics [1-3]. The computer generated hologram (CGH) is also used to generate arbitrary light patterns and has been used for optical manipulation and fabrication because of its high diffraction efficiency and axial confinement. We model the effect of the turbid media as a phase randomization process. We compare the quality and asses the degradation of the projected light pattern for both techniques as it propagates in the turbid media.
Original languageEnglish
Title of host publication546. WE Hereaus Seminar: Light in disordered photonics media
Number of pages1
Place of PublicationGermany
Publication date2013
Publication statusPublished - 2013
Event546. WE Hereaus Seminar: Light in disordered photonics media - Bad Honnef, Germany
Duration: 2 Dec 20134 Dec 2013
Conference number: 546
http://www.we-heraeus-stiftung.de/index.php?option=com_icagenda&view=list&layout=event&id=32&Itemid=547

Conference

Conference546. WE Hereaus Seminar: Light in disordered photonics media
Number546
CountryGermany
CityBad Honnef
Period02/12/201304/12/2013
Internet address

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