Comparative Transcriptomic Analysis of Streptococcus thermophilus TH1436 and TH1477 Showing Different Capability in the Use o f Galactose

Sabrina Giaretta, Laura Treu, Veronica Vendramin, Vinicius da Silva Duarte, Armin Tarrah, Stefano Campanaro, Viviana Corich*, Alessio Giacomini

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

186 Downloads (Pure)

Abstract

Streptococcus thermophilus is a species widely used in the dairy industry for its capability to rapidly ferment lactose and lower the pH. The capability to use galactose produced from lactose hydrolysis is strain dependent and most of commercial S. thermophilus strains are galactose-negative (Gal(-)), although galactose-positive (Gal(+)) would be more technologically advantageous because this feature could provide additional metabolic products and prevent galactose accumulation in foods. In this study, a next generation sequencing transcriptome approach was used to compare for the first time a Gal(+) and a Gal(-) strain to characterize their whole metabolism and shed light on their different properties, metabolic performance and gene regulation. Transcriptome analysis revealed that all genes of the gal operon were expressed very differently in Gal(+) and in the Gal(-) strains. The expression of several genes involved in mixed acid fermentation, PTS sugars transporter and stress response were found enhanced in Gal(+). Conversely, genes related to amino acids, proteins metabolism and CRISPR associated proteins were under-expressed. In addition, the strains showed a diverse series of predicted genes controlled by the transcriptional factor catabolite control protein A (CcpA). Overall, transcriptomic analysis suggests that the Gal+ strain underwent a metabolic remodeling to cope with the changed environmental conditions.
Original languageEnglish
Article number1765
JournalFrontiers in Microbiology
Volume9
Number of pages15
ISSN1664-302X
DOIs
Publication statusPublished - 2018

Keywords

  • CcpA
  • Galactose metabolism
  • RNA-seq
  • Comparative transcriptome analysis
  • Gal-lac operon
  • Mixed acid fermentation

Fingerprint Dive into the research topics of 'Comparative Transcriptomic Analysis of Streptococcus thermophilus TH1436 and TH1477 Showing Different Capability in the Use o f Galactose'. Together they form a unique fingerprint.

Cite this