Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice

Payal B. Watchmaker, Katharina Lahl, Mike Lee, Dirk Baumjohann, John Morton, Sun Jung Kim, Ruizhu Zeng, Alexander Dent, K. Mark Anse, Betty Diamond, Husein Hadeiba, Eugene C. Butcher

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Dendritic cells (DCs) that orchestrate mucosal immunity have been studied in mice. Here we characterized human gut DC populations and defined their relationship to previously studied human and mouse DCs. CD103(+)Sirp alpha(-) DCs were related to human blood CD141(+) DCs and to mouse intestinal CD103(+)CD11b(-) DCs and expressed markers of cross-presenting DCs. CD103(+)Sirp alpha(+) DCs aligned with human blood CD1c(+) DCs and mouse intestinal CD103(+)CD11b(+) DCs and supported the induction of regulatory T cells. Both CD103(+) DC subsets induced the T(H)17 subset of helper T cells, while CD103-Sirp alpha(+) DCs induced the T(H)17 subset of helper T cells. Comparative analysis of transcriptomes revealed conserved transcriptional programs among CD103(+) DC subsets and identified a selective role for the transcriptional repressors BcI-6 and Blimp-1 in the specification of CD103(+)CD11b(-) DCs and intestinal CD103(+)CD11b(+) DCs, respectively. Our results highlight evolutionarily conserved and divergent programming of intestinal DCs.
Original languageEnglish
JournalNature Immunology
Volume15
Issue number1
Pages (from-to)98-108
Number of pages11
ISSN1529-2908
DOIs
Publication statusPublished - 2014
Externally publishedYes

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