Comparative studies of Danish Flavobacterium psychrophilum isolates: ribotypes, plasmid profiles, serotypes and virulence

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Abstract

Ribotyping and plasmid profiling were carried out on 299 Danish Flavobacterium psychrophilum isolates from farmed rainbow trout, Oncorhynchus mykiss (Walbaum). The isolates had been characterized biochemically and serologically in another study. The isolates were very homogeneous, 254 isolates had the same ribotype A (restriction enzyme EcoRI) and 284 isolates harboured one 3.3 kb plasmid. Seventy-five per cent of the F. psychrophilum isolates had ribotype A, one 3.3 kb plasmid and belonged to either serotype Th or Fd. Virulence studies with representatives of the dominant groups classified such isolates as virulent, and an extra small or large plasmid did not change the virulence level. A relationship between the serotypes Fd and Th, certain ribotypes, and virulence was found. The isolates belonging to serotype Fp(T) and to ribotype B were less virulent. Only a few isolates with other ribotypes were found and these were also less virulent. Isolates that did not have the 3.3 kb plasmid were also less virulent. The virulent isolates all harboured the 3.3 kb plasmid; however, there was no clear correlation between virulence and plasmid content as a 3.3 kb plasmid was also found in less virulent isolates.
Original languageEnglish
JournalJournal of Fish Diseases
Volume23
Issue number3
Pages (from-to)211-218
ISSN0140-7775
Publication statusPublished - 2000

Cite this

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title = "Comparative studies of Danish Flavobacterium psychrophilum isolates: ribotypes, plasmid profiles, serotypes and virulence",
abstract = "Ribotyping and plasmid profiling were carried out on 299 Danish Flavobacterium psychrophilum isolates from farmed rainbow trout, Oncorhynchus mykiss (Walbaum). The isolates had been characterized biochemically and serologically in another study. The isolates were very homogeneous, 254 isolates had the same ribotype A (restriction enzyme EcoRI) and 284 isolates harboured one 3.3 kb plasmid. Seventy-five per cent of the F. psychrophilum isolates had ribotype A, one 3.3 kb plasmid and belonged to either serotype Th or Fd. Virulence studies with representatives of the dominant groups classified such isolates as virulent, and an extra small or large plasmid did not change the virulence level. A relationship between the serotypes Fd and Th, certain ribotypes, and virulence was found. The isolates belonging to serotype Fp(T) and to ribotype B were less virulent. Only a few isolates with other ribotypes were found and these were also less virulent. Isolates that did not have the 3.3 kb plasmid were also less virulent. The virulent isolates all harboured the 3.3 kb plasmid; however, there was no clear correlation between virulence and plasmid content as a 3.3 kb plasmid was also found in less virulent isolates.",
keywords = "Fiskesundhed",
author = "Lone Madsen and Inger Dalsgaard",
year = "2000",
language = "English",
volume = "23",
pages = "211--218",
journal = "Journal of Fish Diseases",
issn = "0140-7775",
publisher = "Wiley-Blackwell",
number = "3",

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Comparative studies of Danish Flavobacterium psychrophilum isolates: ribotypes, plasmid profiles, serotypes and virulence. / Madsen, Lone; Dalsgaard, Inger.

In: Journal of Fish Diseases, Vol. 23, No. 3, 2000, p. 211-218.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Comparative studies of Danish Flavobacterium psychrophilum isolates: ribotypes, plasmid profiles, serotypes and virulence

AU - Madsen, Lone

AU - Dalsgaard, Inger

PY - 2000

Y1 - 2000

N2 - Ribotyping and plasmid profiling were carried out on 299 Danish Flavobacterium psychrophilum isolates from farmed rainbow trout, Oncorhynchus mykiss (Walbaum). The isolates had been characterized biochemically and serologically in another study. The isolates were very homogeneous, 254 isolates had the same ribotype A (restriction enzyme EcoRI) and 284 isolates harboured one 3.3 kb plasmid. Seventy-five per cent of the F. psychrophilum isolates had ribotype A, one 3.3 kb plasmid and belonged to either serotype Th or Fd. Virulence studies with representatives of the dominant groups classified such isolates as virulent, and an extra small or large plasmid did not change the virulence level. A relationship between the serotypes Fd and Th, certain ribotypes, and virulence was found. The isolates belonging to serotype Fp(T) and to ribotype B were less virulent. Only a few isolates with other ribotypes were found and these were also less virulent. Isolates that did not have the 3.3 kb plasmid were also less virulent. The virulent isolates all harboured the 3.3 kb plasmid; however, there was no clear correlation between virulence and plasmid content as a 3.3 kb plasmid was also found in less virulent isolates.

AB - Ribotyping and plasmid profiling were carried out on 299 Danish Flavobacterium psychrophilum isolates from farmed rainbow trout, Oncorhynchus mykiss (Walbaum). The isolates had been characterized biochemically and serologically in another study. The isolates were very homogeneous, 254 isolates had the same ribotype A (restriction enzyme EcoRI) and 284 isolates harboured one 3.3 kb plasmid. Seventy-five per cent of the F. psychrophilum isolates had ribotype A, one 3.3 kb plasmid and belonged to either serotype Th or Fd. Virulence studies with representatives of the dominant groups classified such isolates as virulent, and an extra small or large plasmid did not change the virulence level. A relationship between the serotypes Fd and Th, certain ribotypes, and virulence was found. The isolates belonging to serotype Fp(T) and to ribotype B were less virulent. Only a few isolates with other ribotypes were found and these were also less virulent. Isolates that did not have the 3.3 kb plasmid were also less virulent. The virulent isolates all harboured the 3.3 kb plasmid; however, there was no clear correlation between virulence and plasmid content as a 3.3 kb plasmid was also found in less virulent isolates.

KW - Fiskesundhed

M3 - Journal article

VL - 23

SP - 211

EP - 218

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

SN - 0140-7775

IS - 3

ER -