Comparative Genomics of Bacteriophage of the Genus Seuratvirus

Pavelas Sazinas, Tamsin Redgwell, Branko Rihtman, Aurelija Grigonyte, Slawomir Michniewski, David J. Scanlan, Jon Hobman, Andrew Millard*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Despite being more abundant and having smaller genomes than their bacterial host, relatively few bacteriophages have had their genomes sequenced. Here, we isolated 14 bacteriophages from cattle slurry and performed de novo genome sequencing, assembly, and annotation. The commonly used marker genes polB and terL showed these bacteriophages to be closely related to members of the genus Seuratvirus. We performed a core-gene analysis using the 14 new and four closely related genomes. A total of 58 core genes were identified, the majority of which has no known function. These genes were used to construct a core-gene phylogeny, the results of which confirmed the new isolates to be part of the genus Seuratvirus and expanded the number of species within this genus to four. All bacteriophages within the genus contained the genes queCDE encoding enzymes involved in queuosine biosynthesis. We suggest these genes are carried as a mechanism to modify DNA in order to protect these bacteriophages against host endonucleases.
Original languageEnglish
JournalGenome Biology and Evolution
Volume10
Issue number1
Pages (from-to)72-76
Number of pages5
ISSN1759-6653
DOIs
Publication statusPublished - 2017

Bibliographical note

The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • bacteriophage
  • evolution
  • genomics

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