Comparative genomic analyses of β-lactamase (blaCMY-42)-encoding plasmids isolated from wastewater treatment plants in Canada

Adeyinka O. Ajayi, Benjamin J. Perry, Christopher K. Yost*, Rob C. Jamieson, Lisbeth Truelstrup Hansen, Teddie O. Rahube*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Wastewater treatment plants (WWTPs) are useful environments for investigating the occurrence, diversity, and evolution of plasmids encoding clinically relevant antibiotic resistance genes (ARGs). Our objective was to isolate and sequence plasmids encoding meropenem resistance from bacterial hosts within Canadian WWTPs. We used two enrichment culture approaches for primary plasmid isolation, followed by screening for antibiotic resistance, conjugative mobility, and stability in enteric bacteria. Isolated plasmids were sequenced using Illumina MiSeq and Sanger sequencing methods. Bioinformatics analyses resolved a multi-resistance IncF/MOBF12 plasmid, pFEMG (209 357 bp), harbouring resistance genes to β-lactam (blaCMY-42, blaTEM-1β, and blaNDM-5), macrolide (mphA-mrx-mphR), tetracycline (tetR-tetB-tetC-tetD), trimethoprim (dfrA12), aminoglycoside (aadA2), and sulfonamide (sul1) antibiotic classes. We also isolated an IncI1/MOBP12 plasmid pPIMR (172,280 bp) carrying similar β-lactamase and a small multi-drug efflux resistance gene cluster (blaCMY-42-blc-sugE) to pFEMG. The co-occurrence of different ARGs within a single 24,552 bp cluster in pFEMG - interspersed with transposons, insertion sequence elements, and a class 1 integron - may be of significant interest to human and veterinary medicine. Additionally, the presence of conjugative and plasmid maintenance genes in the studied plasmids corresponded to observed high conjugative transfer frequencies and stable maintenance. Extensive investigation is required to further understand the fitness trade-offs of plasmids with different types of conjugative transfer and maintenance modules.
Original languageEnglish
JournalCanadian Journal of Microbiology
Volume67
Issue number10
Pages (from-to)737-748
Number of pages12
ISSN0008-4166
DOIs
Publication statusPublished - 2021

Keywords

  • Wastewater
  • blaCMY-42
  • Resistance plasmids
  • β-lactamase
  • Anti-Bacterial Agents

Fingerprint

Dive into the research topics of 'Comparative genomic analyses of β-lactamase (blaCMY-42)-encoding plasmids isolated from wastewater treatment plants in Canada'. Together they form a unique fingerprint.

Cite this