Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus

E. Brocchi, I.E. Bergmann, A. Dekker, D.J. Paton, D.J. Sammin, Matthias Greiner, S. Grazioli, F. De Simone, H. Yadin, B. Haas, N. Bulut, V. Malirat, E. Neitzert, N. Goris, S. Parida, K. Sørensen, K. De Clercq

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    To validate the use of serology in substantiating freedom from infection after foot-and-mouth disease (FMD) outbreaks have been controlled by measures that include vaccination, 3551 sera were tested with six assays that detect antibodies to the non-structural proteins of FMD virus. The sera came from naive, vaccinated, infected and vaccinated-and-infected animals; two-thirds from cattle, the remainder from sheep and pigs. The assays were covariant for sensitivity, but not necessarily for specificity. A commercial kit from Cedi-diagnostics and an in-house assay from IZS-Brescia were comparable to the NCPanaftosa-screening index method described in the Diagnostic Manual of the World Animal Health Organisation. Using these three tests the specificity and sensitivity for the detection of carriers in vaccinated cattle approaches or exceeds 99% and 90%, respectively.
    Original languageEnglish
    JournalVaccine
    Volume24
    Issue number47-48
    Pages (from-to)6966-6979
    ISSN0264-410X
    Publication statusPublished - 2006

    Keywords

    • tests performances
    • non-structural proteins ELISAs
    • foot-and-mouth disease

    Cite this

    Brocchi, E., Bergmann, I. E., Dekker, A., Paton, D. J., Sammin, D. J., Greiner, M., ... De Clercq, K. (2006). Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus. Vaccine, 24(47-48), 6966-6979.
    Brocchi, E. ; Bergmann, I.E. ; Dekker, A. ; Paton, D.J. ; Sammin, D.J. ; Greiner, Matthias ; Grazioli, S. ; De Simone, F. ; Yadin, H. ; Haas, B. ; Bulut, N. ; Malirat, V. ; Neitzert, E. ; Goris, N. ; Parida, S. ; Sørensen, K. ; De Clercq, K. / Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus. In: Vaccine. 2006 ; Vol. 24, No. 47-48. pp. 6966-6979.
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    title = "Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus",
    abstract = "To validate the use of serology in substantiating freedom from infection after foot-and-mouth disease (FMD) outbreaks have been controlled by measures that include vaccination, 3551 sera were tested with six assays that detect antibodies to the non-structural proteins of FMD virus. The sera came from naive, vaccinated, infected and vaccinated-and-infected animals; two-thirds from cattle, the remainder from sheep and pigs. The assays were covariant for sensitivity, but not necessarily for specificity. A commercial kit from Cedi-diagnostics and an in-house assay from IZS-Brescia were comparable to the NCPanaftosa-screening index method described in the Diagnostic Manual of the World Animal Health Organisation. Using these three tests the specificity and sensitivity for the detection of carriers in vaccinated cattle approaches or exceeds 99{\%} and 90{\%}, respectively.",
    keywords = "tests performances, non-structural proteins ELISAs, foot-and-mouth disease",
    author = "E. Brocchi and I.E. Bergmann and A. Dekker and D.J. Paton and D.J. Sammin and Matthias Greiner and S. Grazioli and {De Simone}, F. and H. Yadin and B. Haas and N. Bulut and V. Malirat and E. Neitzert and N. Goris and S. Parida and K. S{\o}rensen and {De Clercq}, K.",
    year = "2006",
    language = "English",
    volume = "24",
    pages = "6966--6979",
    journal = "Vaccine",
    issn = "0264-410X",
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    number = "47-48",

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    Brocchi, E, Bergmann, IE, Dekker, A, Paton, DJ, Sammin, DJ, Greiner, M, Grazioli, S, De Simone, F, Yadin, H, Haas, B, Bulut, N, Malirat, V, Neitzert, E, Goris, N, Parida, S, Sørensen, K & De Clercq, K 2006, 'Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus', Vaccine, vol. 24, no. 47-48, pp. 6966-6979.

    Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus. / Brocchi, E.; Bergmann, I.E.; Dekker, A.; Paton, D.J.; Sammin, D.J.; Greiner, Matthias; Grazioli, S.; De Simone, F.; Yadin, H.; Haas, B.; Bulut, N.; Malirat, V.; Neitzert, E.; Goris, N.; Parida, S.; Sørensen, K.; De Clercq, K.

    In: Vaccine, Vol. 24, No. 47-48, 2006, p. 6966-6979.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus

    AU - Brocchi, E.

    AU - Bergmann, I.E.

    AU - Dekker, A.

    AU - Paton, D.J.

    AU - Sammin, D.J.

    AU - Greiner, Matthias

    AU - Grazioli, S.

    AU - De Simone, F.

    AU - Yadin, H.

    AU - Haas, B.

    AU - Bulut, N.

    AU - Malirat, V.

    AU - Neitzert, E.

    AU - Goris, N.

    AU - Parida, S.

    AU - Sørensen, K.

    AU - De Clercq, K.

    PY - 2006

    Y1 - 2006

    N2 - To validate the use of serology in substantiating freedom from infection after foot-and-mouth disease (FMD) outbreaks have been controlled by measures that include vaccination, 3551 sera were tested with six assays that detect antibodies to the non-structural proteins of FMD virus. The sera came from naive, vaccinated, infected and vaccinated-and-infected animals; two-thirds from cattle, the remainder from sheep and pigs. The assays were covariant for sensitivity, but not necessarily for specificity. A commercial kit from Cedi-diagnostics and an in-house assay from IZS-Brescia were comparable to the NCPanaftosa-screening index method described in the Diagnostic Manual of the World Animal Health Organisation. Using these three tests the specificity and sensitivity for the detection of carriers in vaccinated cattle approaches or exceeds 99% and 90%, respectively.

    AB - To validate the use of serology in substantiating freedom from infection after foot-and-mouth disease (FMD) outbreaks have been controlled by measures that include vaccination, 3551 sera were tested with six assays that detect antibodies to the non-structural proteins of FMD virus. The sera came from naive, vaccinated, infected and vaccinated-and-infected animals; two-thirds from cattle, the remainder from sheep and pigs. The assays were covariant for sensitivity, but not necessarily for specificity. A commercial kit from Cedi-diagnostics and an in-house assay from IZS-Brescia were comparable to the NCPanaftosa-screening index method described in the Diagnostic Manual of the World Animal Health Organisation. Using these three tests the specificity and sensitivity for the detection of carriers in vaccinated cattle approaches or exceeds 99% and 90%, respectively.

    KW - tests performances

    KW - non-structural proteins ELISAs

    KW - foot-and-mouth disease

    M3 - Journal article

    VL - 24

    SP - 6966

    EP - 6979

    JO - Vaccine

    JF - Vaccine

    SN - 0264-410X

    IS - 47-48

    ER -

    Brocchi E, Bergmann IE, Dekker A, Paton DJ, Sammin DJ, Greiner M et al. Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus. Vaccine. 2006;24(47-48):6966-6979.