Faecal samples originating from fifteen foxes experimentally infected with Echinococcus multilocularis, (0-90 days post inoculation (dpi) were examined by means of coproantigen-ELISA, egg isolation by sieving, PCR on DNA extracted from sieved material (sieve-PCR) and PCR on DNA directly extracted from faeces (direct-PCR). A new egg counting system was developed by combining steps from sieving and McMaster techniques. In comparison with the classical McMaster technique, the new modified system had 32% higher counts in samples from the high patent period, and was also able to detected eggs in samples below the threshold of the classical McMaster method. The egg isolation method detected 83% of patent infections (95% CI: 69-93). The detection level of the coproantigen-ELISA test during the pre-patent period was 63% (95%, CI: 41-81), while it was 47% (95%, CI: 30-65) in the whole patent period. The highest copro-antigen levels were observed at the late pre-patent and early patent periods, declining from mid-patency, with increasing numbers of negative samples towards the end of patency. The direct-PCR method developed in this study detected 16% (95%, CI: 5-36) of samples from pre-patent period and 56% (95%, CI: 38-72) of samples from patent periods. Although addition of BSA improved the detection level of the direct-PCR in the low patent period from 40% to 47%, still prominent inhibition was observed. The detection level of the sieve-PCR test in the whole patent period was 85% (95%, CI: 70-94). This method enabled for the first time the detection of E. multilocularis DNA in the prepatent period but with low sensitivity (19%). DNA from samples with one or even no eggs in 2g of faeces from the patent period was also amplified. The sieve-PCR proved to be the most sensitive method when applied on samples from any period of the infection. This method can be used as a screening method in large-scale epidemiological surveys in low or non-endemic areas and also as a one-step diagnostic test in individual cases.
|Number of pages||1|
|Publication status||Published - 2006|
|Event||Joint Spring Symposium 2006 : Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health - Frederiksberg, Denmark|
Duration: 31 Mar 2006 → …
|Conference||Joint Spring Symposium 2006 : Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health|
|Period||31/03/2006 → …|