Comparative chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357)

Christian Rank; Marie Louise Klejnstrup; Lene Maj Petersen; Sara Kildgaard; Jens Christian Frisvad; Charlotte Held Gotfredsen; Thomas Ostenfeld Larsen

doi:10.3390/metabo2010039

Comparative chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357)

Christian Rank, Marie Louise Klejnstrup, Lene Maj Petersen, Sara Kildgaard, Jens Christian Frisvad, Charlotte Held Gotfredsen, Thomas Ostenfeld Larsen

Department of Chemistry

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Abstract

Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.

Original language	English
Journal	Metabolites
Volume	2
Pages (from-to)	39-56
ISSN	2218-1989
DOIs	https://doi.org/10.3390/metabo2010039
Publication status	Published - 2012

Keywords

Aspergillus oryzae
(RIB40)
Aspergillus flavus
(NRRL 3357)
parasiticolide
ditryptoleucine
oryzamide

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title = "Comparative chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357)",

abstract = "Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.",

keywords = "Aspergillus oryzae, (RIB40), Aspergillus flavus, (NRRL 3357), parasiticolide, ditryptoleucine, oryzamide",

author = "Christian Rank and Klejnstrup, {Marie Louise} and Petersen, {Lene Maj} and Sara Kildgaard and Frisvad, {Jens Christian} and Gotfredsen, {Charlotte Held} and Larsen, {Thomas Ostenfeld}",

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doi = "10.3390/metabo2010039",

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T1 - Comparative chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357)

AU - Rank, Christian

AU - Klejnstrup, Marie Louise

AU - Petersen, Lene Maj

AU - Kildgaard, Sara

AU - Frisvad, Jens Christian

AU - Gotfredsen, Charlotte Held

AU - Larsen, Thomas Ostenfeld

PY - 2012

Y1 - 2012

N2 - Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.

AB - Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.

KW - Aspergillus oryzae

KW - (RIB40)

KW - Aspergillus flavus

KW - (NRRL 3357)

KW - parasiticolide

KW - ditryptoleucine

KW - oryzamide

U2 - 10.3390/metabo2010039

DO - 10.3390/metabo2010039

M3 - Journal article

C2 - 24957367

SN - 2218-1989

VL - 2

SP - 39

EP - 56

JO - Metabolites

JF - Metabolites

ER -

Comparative chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357)

Abstract

Keywords

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