TY - JOUR
T1 - Comparative assay of fluorescent antibody test results among twelve European National Reference Laboratories using various anti-rabies conjugates
AU - Robardet, E.
AU - Andrieu, S.
AU - Rasmussen, Thomas Bruun
AU - Dobrostana, M.
AU - Horton, D. L.
AU - Hostnik, P.
AU - Jaceviciene, I.
AU - Juhasz, T.
AU - Müller, T.
AU - F. Mutinelli,
AU - Servat, A.
AU - M. Smreczak,
AU - Vanek, E.
AU - S. Vázquez-Morón,
AU - Cliquet, F.
PY - 2013
Y1 - 2013
N2 - Twelve National Reference Laboratories (NRLs) for rabies have undertaken a comparative assay to assess the comparison of fluorescent antibody test (FAT) results using five coded commercial anti-rabies conjugates (Biorad, Bioveta, Fujirebio, Millipore, and SIFIN conjugates). Homogenized positive brain tissues infected with various lyssavirus species as well as negative samples were analyzed blindly using a standardized FAT procedure. Conjugates B, C, D, and E were found to be significantly more effective than conjugate A for GS7 (French RABV) diluted samples (1/8 and 1/100) while the frequency of concordant results of conjugates C and D differ significantly from conjugates A, B and E for CVS 27. For detection of EBLV-1 strains, conjugates C and D also presented a significantly lower frequency of discordant results compared to conjugates A, B and E. Conjugates B, C and D were found to be significantly more effective than conjugates E and A for EBLV-2 and ABLV samples. In view of these results, conjugates C and D set themselves apart from the others and appeared as the most effective of this 5-panel conjugates. This study clearly demonstrates that the variability of conjugates used by National Reference Laboratories can potentially lead to discordant results and influence assay sensitivity. In case of false negative results this could have a dramatic impact if the animal under investigation is responsible for human exposure. To avoid such situations, confirmatory tests should be implemented.
AB - Twelve National Reference Laboratories (NRLs) for rabies have undertaken a comparative assay to assess the comparison of fluorescent antibody test (FAT) results using five coded commercial anti-rabies conjugates (Biorad, Bioveta, Fujirebio, Millipore, and SIFIN conjugates). Homogenized positive brain tissues infected with various lyssavirus species as well as negative samples were analyzed blindly using a standardized FAT procedure. Conjugates B, C, D, and E were found to be significantly more effective than conjugate A for GS7 (French RABV) diluted samples (1/8 and 1/100) while the frequency of concordant results of conjugates C and D differ significantly from conjugates A, B and E for CVS 27. For detection of EBLV-1 strains, conjugates C and D also presented a significantly lower frequency of discordant results compared to conjugates A, B and E. Conjugates B, C and D were found to be significantly more effective than conjugates E and A for EBLV-2 and ABLV samples. In view of these results, conjugates C and D set themselves apart from the others and appeared as the most effective of this 5-panel conjugates. This study clearly demonstrates that the variability of conjugates used by National Reference Laboratories can potentially lead to discordant results and influence assay sensitivity. In case of false negative results this could have a dramatic impact if the animal under investigation is responsible for human exposure. To avoid such situations, confirmatory tests should be implemented.
KW - Rabies
KW - Diagnosis
KW - Fluorescent antibody test
KW - Fluorescein isothiocyanate
KW - Conjugate
U2 - 10.1016/j.jviromet.2013.03.027
DO - 10.1016/j.jviromet.2013.03.027
M3 - Journal article
C2 - 23578701
SN - 0166-0934
VL - 191
SP - 88
EP - 94
JO - Journal of Virological Methods
JF - Journal of Virological Methods
ER -