Commodity Chemicals From Engineered Modular Type I Polyketide Synthases

Satoshi Yuzawa, Amin Zargar, Bo Pang, Leonard Katz, Jay D. Keasling

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Reduced polyketides are a subclass of natural products that have a variety of medical, veterinary, and agricultural applications and are well known for their structural diversity. Although these compounds do not resemble each other, they are all made by a class of enzymes known as modular polyketide synthases (PKSs). The commonality of PKS domains/modules that compose PKSs and the understanding of the relationship between the sequence of the PKS and the structure of the compound it produces render modular PKSs as excellent targets for engineering to produce novel compounds with predicted structures. Here, we describe experimental protocols and considerations for modular PKS engineering and two case studies to produce commodity chemicals by engineered PKSs.
Original languageEnglish
Title of host publicationMethods in Enzymology : Enzymes in Synthetic Biology
Volume608
PublisherElsevier
Publication date2018
Pages393-415
Chapter14
ISBN (Electronic)9780128151495
DOIs
Publication statusPublished - 2018
SeriesMethods in Enzymology
ISSN0076-6879

Keywords

  • Commodity chemicals
  • Metabolic engineering
  • Polyketide synthases
  • Protein engineering
  • Synthetic biology

Cite this

Yuzawa, S., Zargar, A., Pang, B., Katz, L., & Keasling, J. D. (2018). Commodity Chemicals From Engineered Modular Type I Polyketide Synthases. In Methods in Enzymology: Enzymes in Synthetic Biology (Vol. 608, pp. 393-415). Elsevier. Methods in Enzymology https://doi.org/10.1016/bs.mie.2018.04.027