Commercial and in-house RT-PCR methods for detection of PRRSV. Design, performance and pitfalls

Charlotte Kristiane Hjulsager

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    RT-PCR is a widely used method for detection of PRRSV, because it is a rapid, sensitive and highly specific detection tool. However, the profound diversity and rapid evolution of PRRSV genomes complicates the development of highly sensitive and robust assays. Several factors need to be taken into consideration when using RT-PCR for diagnosis of PRRSV.
    Original languageEnglish
    Title of host publicationProceedings of EuroPRRS2012 : Understanding and combating PRRS in Europe
    Place of PublicationBudapest, Hungary
    Publication date2012
    Pages20-27
    ISBN (Print)978-963-269-312-5
    Publication statusPublished - 2012
    Event3rd EuroPRRSnet conference on Understanding and Combatting PRRS in Europe - Danubius Health Spa Resort and Grand Hotel, Budapest, Hungary
    Duration: 10 Oct 201212 Oct 2012
    http://www.europrrs.net/events/europrrs2012/index.html

    Conference

    Conference3rd EuroPRRSnet conference on Understanding and Combatting PRRS in Europe
    LocationDanubius Health Spa Resort and Grand Hotel
    CountryHungary
    CityBudapest
    Period10/10/201212/10/2012
    Internet address

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