Comet assay on mice testicular cells

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Heritable mutations may result in a variety of adverse outcomes including genetic disease in the offspring. In recent years the focus on germ cell mutagenicity has increased and the “Globally Harmonized System of Classification and Labelling of Chemicals (GHS)” has published classification criteria for germ cell mutagens (Speit et al., 2009). The in vivo Comet assay is considered a useful tool for investigating germ cell genotoxicity. In the present study DNA strand breaks in testicular cells of mice were investigated. Different classes of chemicals were tested in order to evaluate the sensitivity of the comet assay in testicular cells. The chemicals included environmentally relevant substances such as Bisphenol A, PFOS and Tetrabrombisphenol A. Statistical power calculations will be presented to aid in the design of future Comet assay studies on testicular cells. Power curves were provided with different fold changes in % tail DNA, different number of cells scored and different number of gels (Hansen et al., 2014). An example is shown in Figure 1. A high throughput version of the Comet assay was used. Samples were scored with a fully automatic comet assay scoring system that provided faster scoring of randomly selected cells.
Original languageEnglish
Title of host publicationICAW 2015 - 11th International Comet Assay Workshop
Number of pages1
Place of PublicationAntwerpen, Belgium
Publication date2015
Publication statusPublished - 2015
EventICAW 2015 - 11th International Comet Assay Workshop - Antwerpen, Belgium
Duration: 1 Sep 20154 Sep 2015


ConferenceICAW 2015 - 11th International Comet Assay Workshop

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