Combined effects of microplastics and chemical contaminants on the organ toxicity of zebrafish ( Danio rerio )

Sandra Rainieri*, Nadia Conlledo, Bodil Katrine Larsen, Kit Granby, Alejandro Barranco

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Microplastics contamination of the aquatic environment is considered a growing problem. The ingestion of microplastics has been documented for a variety of aquatic animals. Studies have shown the potential of microplastics to affect the bioavailability and uptake route of sorbed co-contaminants of different nature in living organisms. Persistent organic pollutants and metals have been the co-contaminants majorly investigated in this field. The combined effect of microplastics and sorbed co-contaminants in aquatic organisms still needs to be properly understood. To address this, we have subjected zebrafish to four different feeds: A) untreated feed; B) feed supplemented with microplastics (LD-PE 125–250 µm of diameter); C) feed supplemented with 2% microplastics to which a mixture of PCBs, BFRs, PFCs and methylmercury were sorbed; and D) feed supplemented with the mixture of contaminants only. After 3 weeks of exposure fish were dissected and liver, intestine, muscular tissue and brain were extracted. After visual observation, evaluation of differential gene expression of some selected biomarker genes in liver, intestine and brain were carried out. Additionally, quantification of perfluorinated compounds in liver, brain, muscular tissue and intestine of some selected samples were performed. The feed supplemented with microplastics with sorbed contaminants produced the most evident effects especially on the liver. The results indicate that microplastics alone does not produce relevant effects on zebrafish in the experimental conditions tested; on the contrary, the combined effect of microplastics and sorbed contaminants altered significantly their organs homeostasis in a greater manner than the contaminants alone.
Original languageEnglish
JournalEnvironmental Research
Volume162
Pages (from-to)135-143
ISSN0013-9351
DOIs
Publication statusPublished - 2018

Keywords

  • Gene expression
  • Microplastics
  • Perfluorinated compounds
  • Toxicity
  • Sorbed contaminants
  • Zebrafish

Cite this

Rainieri, Sandra ; Conlledo, Nadia ; Larsen, Bodil Katrine ; Granby, Kit ; Barranco, Alejandro. / Combined effects of microplastics and chemical contaminants on the organ toxicity of zebrafish ( Danio rerio ). In: Environmental Research. 2018 ; Vol. 162. pp. 135-143.
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author = "Sandra Rainieri and Nadia Conlledo and Larsen, {Bodil Katrine} and Kit Granby and Alejandro Barranco",
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Combined effects of microplastics and chemical contaminants on the organ toxicity of zebrafish ( Danio rerio ). / Rainieri, Sandra; Conlledo, Nadia; Larsen, Bodil Katrine; Granby, Kit; Barranco, Alejandro.

In: Environmental Research, Vol. 162, 2018, p. 135-143.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Combined effects of microplastics and chemical contaminants on the organ toxicity of zebrafish ( Danio rerio )

AU - Rainieri, Sandra

AU - Conlledo, Nadia

AU - Larsen, Bodil Katrine

AU - Granby, Kit

AU - Barranco, Alejandro

PY - 2018

Y1 - 2018

N2 - Microplastics contamination of the aquatic environment is considered a growing problem. The ingestion of microplastics has been documented for a variety of aquatic animals. Studies have shown the potential of microplastics to affect the bioavailability and uptake route of sorbed co-contaminants of different nature in living organisms. Persistent organic pollutants and metals have been the co-contaminants majorly investigated in this field. The combined effect of microplastics and sorbed co-contaminants in aquatic organisms still needs to be properly understood. To address this, we have subjected zebrafish to four different feeds: A) untreated feed; B) feed supplemented with microplastics (LD-PE 125–250 µm of diameter); C) feed supplemented with 2% microplastics to which a mixture of PCBs, BFRs, PFCs and methylmercury were sorbed; and D) feed supplemented with the mixture of contaminants only. After 3 weeks of exposure fish were dissected and liver, intestine, muscular tissue and brain were extracted. After visual observation, evaluation of differential gene expression of some selected biomarker genes in liver, intestine and brain were carried out. Additionally, quantification of perfluorinated compounds in liver, brain, muscular tissue and intestine of some selected samples were performed. The feed supplemented with microplastics with sorbed contaminants produced the most evident effects especially on the liver. The results indicate that microplastics alone does not produce relevant effects on zebrafish in the experimental conditions tested; on the contrary, the combined effect of microplastics and sorbed contaminants altered significantly their organs homeostasis in a greater manner than the contaminants alone.

AB - Microplastics contamination of the aquatic environment is considered a growing problem. The ingestion of microplastics has been documented for a variety of aquatic animals. Studies have shown the potential of microplastics to affect the bioavailability and uptake route of sorbed co-contaminants of different nature in living organisms. Persistent organic pollutants and metals have been the co-contaminants majorly investigated in this field. The combined effect of microplastics and sorbed co-contaminants in aquatic organisms still needs to be properly understood. To address this, we have subjected zebrafish to four different feeds: A) untreated feed; B) feed supplemented with microplastics (LD-PE 125–250 µm of diameter); C) feed supplemented with 2% microplastics to which a mixture of PCBs, BFRs, PFCs and methylmercury were sorbed; and D) feed supplemented with the mixture of contaminants only. After 3 weeks of exposure fish were dissected and liver, intestine, muscular tissue and brain were extracted. After visual observation, evaluation of differential gene expression of some selected biomarker genes in liver, intestine and brain were carried out. Additionally, quantification of perfluorinated compounds in liver, brain, muscular tissue and intestine of some selected samples were performed. The feed supplemented with microplastics with sorbed contaminants produced the most evident effects especially on the liver. The results indicate that microplastics alone does not produce relevant effects on zebrafish in the experimental conditions tested; on the contrary, the combined effect of microplastics and sorbed contaminants altered significantly their organs homeostasis in a greater manner than the contaminants alone.

KW - Gene expression

KW - Microplastics

KW - Perfluorinated compounds

KW - Toxicity

KW - Sorbed contaminants

KW - Zebrafish

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DO - 10.1016/j.envres.2017.12.019

M3 - Journal article

VL - 162

SP - 135

EP - 143

JO - Environmental Research

JF - Environmental Research

SN - 0013-9351

ER -