Combined Cell Culture-Biosensing Platform Using Vertically Aligned Patterned Peptide Nanofibers for Cellular Studies

Mehmet B. Taskin, Luigi Sasso, Maria Dimaki, Winnie E. Svendsen, Jaime Castillo

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    This Article presents the development of a combined cell culture–biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture–biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.
    Original languageEnglish
    JournalA C S Applied Materials and Interfaces
    Volume5
    Issue number8
    Pages (from-to)3323-3328
    ISSN1944-8244
    DOIs
    Publication statusPublished - 2013

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