Combined Cell Culture-Biosensing Platform Using Vertically Aligned Patterned Peptide Nanofibers for Cellular Studies

Mehmet B. Taskin, Luigi Sasso, Maria Dimaki, Winnie E. Svendsen, Jaime Castillo

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    This Article presents the development of a combined cell culture–biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture–biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.
    Original languageEnglish
    JournalA C S Applied Materials and Interfaces
    Volume5
    Issue number8
    Pages (from-to)3323-3328
    ISSN1944-8244
    DOIs
    Publication statusPublished - 2013

    Cite this

    @article{906307650d3b4a159aae5aeb947bfc88,
    title = "Combined Cell Culture-Biosensing Platform Using Vertically Aligned Patterned Peptide Nanofibers for Cellular Studies",
    abstract = "This Article presents the development of a combined cell culture–biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture–biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.",
    author = "Taskin, {Mehmet B.} and Luigi Sasso and Maria Dimaki and Svendsen, {Winnie E.} and Jaime Castillo",
    year = "2013",
    doi = "10.1021/am400390g",
    language = "English",
    volume = "5",
    pages = "3323--3328",
    journal = "A C S Applied Materials and Interfaces",
    issn = "1944-8244",
    publisher = "American Chemical Society",
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    }

    Combined Cell Culture-Biosensing Platform Using Vertically Aligned Patterned Peptide Nanofibers for Cellular Studies. / Taskin, Mehmet B.; Sasso, Luigi; Dimaki, Maria; Svendsen, Winnie E.; Castillo, Jaime.

    In: A C S Applied Materials and Interfaces, Vol. 5, No. 8, 2013, p. 3323-3328.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Combined Cell Culture-Biosensing Platform Using Vertically Aligned Patterned Peptide Nanofibers for Cellular Studies

    AU - Taskin, Mehmet B.

    AU - Sasso, Luigi

    AU - Dimaki, Maria

    AU - Svendsen, Winnie E.

    AU - Castillo, Jaime

    PY - 2013

    Y1 - 2013

    N2 - This Article presents the development of a combined cell culture–biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture–biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.

    AB - This Article presents the development of a combined cell culture–biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture–biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.

    U2 - 10.1021/am400390g

    DO - 10.1021/am400390g

    M3 - Journal article

    VL - 5

    SP - 3323

    EP - 3328

    JO - A C S Applied Materials and Interfaces

    JF - A C S Applied Materials and Interfaces

    SN - 1944-8244

    IS - 8

    ER -