Abstract
| Original language | English |
|---|---|
| Journal | A C S Applied Materials and Interfaces |
| Volume | 5 |
| Issue number | 8 |
| Pages (from-to) | 3323-3328 |
| ISSN | 1944-8244 |
| DOIs | |
| Publication status | Published - 2013 |
Cite this
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Combined Cell Culture-Biosensing Platform Using Vertically Aligned Patterned Peptide Nanofibers for Cellular Studies. / Taskin, Mehmet B.; Sasso, Luigi; Dimaki, Maria; Svendsen, Winnie E.; Castillo, Jaime.
In: A C S Applied Materials and Interfaces, Vol. 5, No. 8, 2013, p. 3323-3328.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Combined Cell Culture-Biosensing Platform Using Vertically Aligned Patterned Peptide Nanofibers for Cellular Studies
AU - Taskin, Mehmet B.
AU - Sasso, Luigi
AU - Dimaki, Maria
AU - Svendsen, Winnie E.
AU - Castillo, Jaime
PY - 2013
Y1 - 2013
N2 - This Article presents the development of a combined cell culture–biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture–biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.
AB - This Article presents the development of a combined cell culture–biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture–biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.
U2 - 10.1021/am400390g
DO - 10.1021/am400390g
M3 - Journal article
VL - 5
SP - 3323
EP - 3328
JO - A C S Applied Materials and Interfaces
JF - A C S Applied Materials and Interfaces
SN - 1944-8244
IS - 8
ER -