Combined Assay for Detecting Autoantibodies to Nucleic Acids and Apolipoprotein H in Patients with Systemic Lupus Erythematosus

Sangita Khatri*, Elizabeth D. Mellins, Syeda Atia Bukhari, Kira Astakhova, Kathryn S. Torok

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

The complicated clinical picture and biomolecular pattern of human autoimmune diseases (ADs) make knowledge on their etiology still fragmentary. The diagnostic approaches for ADs require improvement both for clinical and research effort to progress. Synthetic biomolecular antigens find growing applications for diagnosis and investigation of ADs. The main goal of this work is to detect interaction between synthetic antigens and autoantibodies in systemic lupus erythematosus within a combined, high-throughput assay. A panel of synthetic antigens has been prepared from DNA, RNA, locked nucleic acids and apolipoprotein H. The binding of synthetic antigens to autoantibodies has been confirmed in sera samples from those with active systemic lupus erythematosus (SLE) by indirect enzyme linked immunosorbent assay. Our study provides an efficient methodology for combined autoantibody profiling in SLE.
Original languageEnglish
Title of host publicationNucleic Acid Detection and Structural Investigations : Methods and Protocols
EditorsKira Astakhova , Syeda Atia Bukhari
Number of pages15
Volume2063
Place of PublicationNew York, United States
PublisherSpringer
Publication date2019
Pages57-71
Chapter6
ISBN (Print)978-1-0716-0137-2
ISBN (Electronic)978-1-0716-0138-9
DOIs
Publication statusPublished - 2019
SeriesMethods in Molecular Biology
ISSN1064-3745

Keywords

  • Autoimmune diseases
  • Antigens
  • Autoantibodies
  • Systemic lupus erythematosus
  • ELISA

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