Combination of phage and Gram-positive bacterial display of human antibody repertoires enables isolation of functional high affinity binders

Francis Jingxin Hu, Anna-Luisa Volk, Helena Persson, Anna Säll, Carl Borrebaeck, Mathias Uhlén, Johan Rockberg*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Surface display couples genotype with a surface exposed phenotype and thereby allows screening of gene-encoded protein libraries for desired characteristics. Of the various display systems available, phage display is by far the most popular, mainly thanks to its ability to harbour large size libraries. Here, we describe the first use of a Gram-positive bacterial host for display of a library of human antibody genes which, when combined with phage display, provides ease of use for screening, sorting and ranking by flow cytometry. We demonstrate the utility of this method by identifying low nanomolar affinity scFv fragments towards human epidermal growth factor receptor 2 (HER2). The ranking and performance of the scFv isolated by flow sorting in surface-immobilised form was retained when expressed as soluble scFv and analysed by biolayer interferometry, as well as after expression as full-length antibodies in mammalian cells. We also demonstrate the possibility of using Gram-positive bacterial display to directly improve the affinity of the identified binders via an affinity maturation step using random mutagenesis and flow sorting. This combined approach has the potential for a more complete scan of the antibody repertoire and for affinity maturation of human antibody formats.
Original languageEnglish
JournalNew Biotechnology
Volume45
Pages (from-to)80-88
Number of pages9
ISSN1871-6784
DOIs
Publication statusPublished - 2018

Bibliographical note

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Keywords

  • Antibody
  • Flow cytometry
  • HER2
  • S. carnosus
  • Affinity maturation
  • Cell-surface display
  • Phage display

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