Combating Microbial Contamination with Robust Polymeric Nanofibers: Elemental Effect on the Mussel-Inspired Cross-Linking of Electrospun Gelatin

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

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Combating Microbial Contamination with Robust Polymeric Nanofibers: Elemental Effect on the Mussel-Inspired Cross-Linking of Electrospun Gelatin. / Leung, Chak Ming; Dhand, Chetna; Dwivedi, Neeraj; Xiao, Amy; Ong, Seow Theng; Chalasani, Madhavi Latha Somaraju; Sriram, Harini; Balakrishnan, Yamini; Dolatshahi-Pirouz, Alireza; Orive, Gorka; Beuerman, Roger Wilmer; Ramakrishna, Seeram; Verma, Navin Kumar; Lakshminarayanan, Rajamani.

In: Acs Applied Bio Materials, Vol. 2, No. 2, 2019, p. 807-823.

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

Harvard

Leung, CM, Dhand, C, Dwivedi, N, Xiao, A, Ong, ST, Chalasani, MLS, Sriram, H, Balakrishnan, Y, Dolatshahi-Pirouz, A, Orive, G, Beuerman, RW, Ramakrishna, S, Verma, NK & Lakshminarayanan, R 2019, 'Combating Microbial Contamination with Robust Polymeric Nanofibers: Elemental Effect on the Mussel-Inspired Cross-Linking of Electrospun Gelatin', Acs Applied Bio Materials, vol. 2, no. 2, pp. 807-823. https://doi.org/10.1021/acsabm.8b00666

APA

CBE

Leung CM, Dhand C, Dwivedi N, Xiao A, Ong ST, Chalasani MLS, Sriram H, Balakrishnan Y, Dolatshahi-Pirouz A, Orive G, Beuerman RW, Ramakrishna S, Verma NK, Lakshminarayanan R. 2019. Combating Microbial Contamination with Robust Polymeric Nanofibers: Elemental Effect on the Mussel-Inspired Cross-Linking of Electrospun Gelatin. Acs Applied Bio Materials. 2(2):807-823. https://doi.org/10.1021/acsabm.8b00666

MLA

Vancouver

Author

Leung, Chak Ming ; Dhand, Chetna ; Dwivedi, Neeraj ; Xiao, Amy ; Ong, Seow Theng ; Chalasani, Madhavi Latha Somaraju ; Sriram, Harini ; Balakrishnan, Yamini ; Dolatshahi-Pirouz, Alireza ; Orive, Gorka ; Beuerman, Roger Wilmer ; Ramakrishna, Seeram ; Verma, Navin Kumar ; Lakshminarayanan, Rajamani. / Combating Microbial Contamination with Robust Polymeric Nanofibers: Elemental Effect on the Mussel-Inspired Cross-Linking of Electrospun Gelatin. In: Acs Applied Bio Materials. 2019 ; Vol. 2, No. 2. pp. 807-823.

Bibtex

@article{4d91b43c6c2b4d0b994aa3e4587b4b3a,
title = "Combating Microbial Contamination with Robust Polymeric Nanofibers: Elemental Effect on the Mussel-Inspired Cross-Linking of Electrospun Gelatin",
abstract = "Designing biocompatible nanofibrous mats capable of preventing microbial colonization from resident and nosocomial bacteria for an extended period remains an unmet clinical need. In the present work, we designed antibiotic free durable antimicrobial nanofiber mats by taking advantage of synergistic interactions between polydopamine(pDA) and metal ions with varying degree of antimicrobial properties (Ag+, Mg2+, Ca2+, and Zn2+). Microscopic analysis showed successful pDA-mediated cross-linking of the gelatin nanofibers, which further improved by the inclusion of Ag+, Mg2+, and Ca2+ ions as supported by mechanical and thermal studies. Spectroscopic results reinforce the presence of strong interactions between pDA and metal ions in the composite nanofibers, leading to generation of robust polymeric nanofibers. We further showed that strong pDA–Ag interactions attenuated the cell cytotoxicity and anticell proliferative properties of silver ions for immortalized keratinocytes and primary human dermal fibroblasts. pDA–Ca2+/Zn2+ interactions rendered the composite structure sterile against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium strains, whereas the silver ion-incorporated composite mats displayed broad spectrum antibacterial activity against both Gram-positive/-negative bacteria and yeast strains. We showed that the strong pDA–Ag interactions help retaining long-term antimicrobial activity of the mats for at least 40 days while attenuating mammalian cell cytotoxicity of silver ions for skin cells. Overall, the results suggest the potential of pDA–metal ion interactions for engineering sterile nanofibrous mats and expanding the antibiotic armamentarium against drug-resistant pathogens.",
keywords = "Antimicrobial, Polydopamine cross-linking, Electrospinning, Gelatin, Metal ions, Tissue engineering",
author = "Leung, {Chak Ming} and Chetna Dhand and Neeraj Dwivedi and Amy Xiao and Ong, {Seow Theng} and Chalasani, {Madhavi Latha Somaraju} and Harini Sriram and Yamini Balakrishnan and Alireza Dolatshahi-Pirouz and Gorka Orive and Beuerman, {Roger Wilmer} and Seeram Ramakrishna and Verma, {Navin Kumar} and Rajamani Lakshminarayanan",
year = "2019",
doi = "10.1021/acsabm.8b00666",
language = "English",
volume = "2",
pages = "807--823",
journal = "Acs Applied Bio Materials",
issn = "2576-6422",
publisher = "American Chemical Society",
number = "2",

}

RIS

TY - JOUR

T1 - Combating Microbial Contamination with Robust Polymeric Nanofibers: Elemental Effect on the Mussel-Inspired Cross-Linking of Electrospun Gelatin

AU - Leung, Chak Ming

AU - Dhand, Chetna

AU - Dwivedi, Neeraj

AU - Xiao, Amy

AU - Ong, Seow Theng

AU - Chalasani, Madhavi Latha Somaraju

AU - Sriram, Harini

AU - Balakrishnan, Yamini

AU - Dolatshahi-Pirouz, Alireza

AU - Orive, Gorka

AU - Beuerman, Roger Wilmer

AU - Ramakrishna, Seeram

AU - Verma, Navin Kumar

AU - Lakshminarayanan, Rajamani

PY - 2019

Y1 - 2019

N2 - Designing biocompatible nanofibrous mats capable of preventing microbial colonization from resident and nosocomial bacteria for an extended period remains an unmet clinical need. In the present work, we designed antibiotic free durable antimicrobial nanofiber mats by taking advantage of synergistic interactions between polydopamine(pDA) and metal ions with varying degree of antimicrobial properties (Ag+, Mg2+, Ca2+, and Zn2+). Microscopic analysis showed successful pDA-mediated cross-linking of the gelatin nanofibers, which further improved by the inclusion of Ag+, Mg2+, and Ca2+ ions as supported by mechanical and thermal studies. Spectroscopic results reinforce the presence of strong interactions between pDA and metal ions in the composite nanofibers, leading to generation of robust polymeric nanofibers. We further showed that strong pDA–Ag interactions attenuated the cell cytotoxicity and anticell proliferative properties of silver ions for immortalized keratinocytes and primary human dermal fibroblasts. pDA–Ca2+/Zn2+ interactions rendered the composite structure sterile against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium strains, whereas the silver ion-incorporated composite mats displayed broad spectrum antibacterial activity against both Gram-positive/-negative bacteria and yeast strains. We showed that the strong pDA–Ag interactions help retaining long-term antimicrobial activity of the mats for at least 40 days while attenuating mammalian cell cytotoxicity of silver ions for skin cells. Overall, the results suggest the potential of pDA–metal ion interactions for engineering sterile nanofibrous mats and expanding the antibiotic armamentarium against drug-resistant pathogens.

AB - Designing biocompatible nanofibrous mats capable of preventing microbial colonization from resident and nosocomial bacteria for an extended period remains an unmet clinical need. In the present work, we designed antibiotic free durable antimicrobial nanofiber mats by taking advantage of synergistic interactions between polydopamine(pDA) and metal ions with varying degree of antimicrobial properties (Ag+, Mg2+, Ca2+, and Zn2+). Microscopic analysis showed successful pDA-mediated cross-linking of the gelatin nanofibers, which further improved by the inclusion of Ag+, Mg2+, and Ca2+ ions as supported by mechanical and thermal studies. Spectroscopic results reinforce the presence of strong interactions between pDA and metal ions in the composite nanofibers, leading to generation of robust polymeric nanofibers. We further showed that strong pDA–Ag interactions attenuated the cell cytotoxicity and anticell proliferative properties of silver ions for immortalized keratinocytes and primary human dermal fibroblasts. pDA–Ca2+/Zn2+ interactions rendered the composite structure sterile against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium strains, whereas the silver ion-incorporated composite mats displayed broad spectrum antibacterial activity against both Gram-positive/-negative bacteria and yeast strains. We showed that the strong pDA–Ag interactions help retaining long-term antimicrobial activity of the mats for at least 40 days while attenuating mammalian cell cytotoxicity of silver ions for skin cells. Overall, the results suggest the potential of pDA–metal ion interactions for engineering sterile nanofibrous mats and expanding the antibiotic armamentarium against drug-resistant pathogens.

KW - Antimicrobial

KW - Polydopamine cross-linking

KW - Electrospinning

KW - Gelatin

KW - Metal ions

KW - Tissue engineering

U2 - 10.1021/acsabm.8b00666

DO - 10.1021/acsabm.8b00666

M3 - Journal article

VL - 2

SP - 807

EP - 823

JO - Acs Applied Bio Materials

JF - Acs Applied Bio Materials

SN - 2576-6422

IS - 2

ER -