Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre

M. Lisby, Uffe Hasbro Mortensen, R. Rothstein

    Research output: Contribution to journalJournal articleResearchpeer-review


    DNA double-strand break repair (DSBR) is an essential process for preserving genomic integrity in all organisms. To investigate this process at the cellular level, we engineered a system of fluorescently marked DNA double-strand breaks (DSBs) in the yeast Saccharomyces cerevisiae to visualize in vivo DSBR in single cells. Using this system, we demonstrate for the first time that Rad52 DNA repair foci and DSBs colocalize. Time-lapse microscopy reveals that the relocalization of Rad52 protein into a focal assembly is a rapid and reversible process. In addition, analysis of DNA damage checkpoint-deficient cells provides direct evidence for coordination between DNA repair and subsequent release from checkpoint arrest. Finally, analyses of cells experiencing multiple DSBs demonstrate that Rad52 foci are centres of DNA repair capable of simultaneously recruiting more than one DSB.
    Original languageEnglish
    JournalNature Cell Biology
    Issue number6
    Pages (from-to)572-577
    Publication statusPublished - 2003


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