Collagenase and Tyrosinase Inhibitory Effect of Isolated Constituents from the Moss Polytrichum formosum

Raíssa Volpatto Marques, Agnès Guillaumin, Ahmed B. Abdelwahab, Aleksander Salwinski, Charlotte H. Gotfredsen, Frédéric Bourgaud, Kasper Enemark-Rasmussen, Sissi Miguel, Henrik Toft Simonsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Mosses from the genus Polytrichum have been shown to contain rare benzonaphthoxanthenones compounds, and many of these have been reported to have important biological activities. In this study, extracts from Polytrichum formosum were analyzed in vitro for their inhibitory properties on collagenase and tyrosinase activity, two important cosmetic target enzymes involved respectively in skin aging and pigmentation. The 70% ethanol extract showed a dose-dependent inhibitory effect against collagenase (IC50 = 4.65 mg/mL). The methanol extract showed a mild inhibitory effect of 44% against tyrosinase at 5.33 mg/mL. Both extracts were investigated to find the constituents having a specific affinity to the enzyme targets collagenase and tyrosinase. The known compounds ohioensin A (1), ohioensin C (3), and communin B (4), together with nor-ohioensin D (2), a new benzonaphthoxanthenone, were isolated from P. formosum. Their structures were determined by mass spectrometry and NMR spectroscopy. Compounds 1 (IC50 = 71.99 µM) and 2 (IC50 = 167.33 µM) showed inhibitory activity against collagenase. Compound 1 also exhibited inhibition of 30% against tyrosinase activity at 200 µM. The binding mode of the active compounds was theoretically generated by an in-silico approach against the 3D structures of collagenase and tyrosinase. These current results present the potential application from the moss P. formosum as a new natural source of collagenase and tyrosinase inhibitors.
Original languageEnglish
Article number1271
Issue number7
Number of pages13
Publication statusPublished - 2021


  • Polytrichum formosum
  • Polytrichaceae
  • Mosses
  • Bryophytes
  • Benzonaphthoxanthenones
  • Ohioensins
  • Collagenase inhibitory activity
  • Tyrosinase inhibitory activity


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