Collagen Type I Improves the Differentiation of Human Embryonic Stem Cells towards Definitive Endoderm

Camilla Holzmann Rasmussen; Dorthe Roenn Petersen; Jonas Bech Møller; Mattias Hansson; Martin Dufva

doi:10.1371/journal.pone.0145389

Collagen Type I Improves the Differentiation of Human Embryonic Stem Cells towards Definitive Endoderm

Camilla Holzmann Rasmussen, Dorthe Roenn Petersen, Jonas Bech Møller, Mattias Hansson, Martin Dufva

Research output: Contribution to journal › Journal article › Research › peer-review

269 Downloads (Pure)

Abstract

Human embryonic stem cells have the ability to generate all cell types in the body and can potentially provide an unlimited source of cells for cell replacement therapy to treat degenerative diseases such as diabetes. Current differentiation protocols of human embryonic stem cells towards insulin producing beta cells focus on soluble molecules whereas the impact of cell-matrix interactions has been mainly unattended. In this study almost 500 different extracellular matrix protein combinations were screened to systemically identify extracellular matrix proteins that influence differentiation of human embryonic stem cells to the definitive endoderm lineage. The percentage of definitive endoderm cells after differentiation on collagen I and fibronectin was >85% and 65%, respectively. The cells on collagen I substrates displayed different morphology and gene expression during differentiation as assessed by time lapse studies compared to cells on the other tested substrates. Global gene expression analysis showed that cells differentiated on collagen I were largely similar to cells on fibronectin after completed differentiation. Collectively, the data suggest that collagen I induces a more rapid and consistent differentiation of stem cells to definitive endoderm. The results shed light on the importance of extracellular matrix proteins for differentiation and also points to a cost effective and easy method to improve differentiation.

Original language	English
Article number	e0145389
Journal	P L o S One
Volume	10
Issue number	12
Number of pages	21
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0145389
Publication status	Published - 2015

Bibliographical note

© 2015 Rasmussen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Access to Document

10.1371/journal.pone.0145389

JournalFinal published version, 2.07 MB

Fingerprint Dive into the research topics of 'Collagen Type I Improves the Differentiation of Human Embryonic Stem Cells towards Definitive Endoderm'. Together they form a unique fingerprint.

Cite this

@article{19a9700ecf5f4eef96420753b0282d11,

title = "Collagen Type I Improves the Differentiation of Human Embryonic Stem Cells towards Definitive Endoderm",

abstract = "Human embryonic stem cells have the ability to generate all cell types in the body and can potentially provide an unlimited source of cells for cell replacement therapy to treat degenerative diseases such as diabetes. Current differentiation protocols of human embryonic stem cells towards insulin producing beta cells focus on soluble molecules whereas the impact of cell-matrix interactions has been mainly unattended. In this study almost 500 different extracellular matrix protein combinations were screened to systemically identify extracellular matrix proteins that influence differentiation of human embryonic stem cells to the definitive endoderm lineage. The percentage of definitive endoderm cells after differentiation on collagen I and fibronectin was >85% and 65%, respectively. The cells on collagen I substrates displayed different morphology and gene expression during differentiation as assessed by time lapse studies compared to cells on the other tested substrates. Global gene expression analysis showed that cells differentiated on collagen I were largely similar to cells on fibronectin after completed differentiation. Collectively, the data suggest that collagen I induces a more rapid and consistent differentiation of stem cells to definitive endoderm. The results shed light on the importance of extracellular matrix proteins for differentiation and also points to a cost effective and easy method to improve differentiation. ",

author = "Rasmussen, {Camilla Holzmann} and Petersen, {Dorthe Roenn} and M{\o}ller, {Jonas Bech} and Mattias Hansson and Martin Dufva",

note = "{\textcopyright} 2015 Rasmussen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2015",

doi = "10.1371/journal.pone.0145389",

language = "English",

volume = "10",

journal = "P L o S One",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "12",

}

TY - JOUR

T1 - Collagen Type I Improves the Differentiation of Human Embryonic Stem Cells towards Definitive Endoderm

AU - Rasmussen, Camilla Holzmann

AU - Petersen, Dorthe Roenn

AU - Møller, Jonas Bech

AU - Hansson, Mattias

AU - Dufva, Martin

N1 - © 2015 Rasmussen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2015

Y1 - 2015

N2 - Human embryonic stem cells have the ability to generate all cell types in the body and can potentially provide an unlimited source of cells for cell replacement therapy to treat degenerative diseases such as diabetes. Current differentiation protocols of human embryonic stem cells towards insulin producing beta cells focus on soluble molecules whereas the impact of cell-matrix interactions has been mainly unattended. In this study almost 500 different extracellular matrix protein combinations were screened to systemically identify extracellular matrix proteins that influence differentiation of human embryonic stem cells to the definitive endoderm lineage. The percentage of definitive endoderm cells after differentiation on collagen I and fibronectin was >85% and 65%, respectively. The cells on collagen I substrates displayed different morphology and gene expression during differentiation as assessed by time lapse studies compared to cells on the other tested substrates. Global gene expression analysis showed that cells differentiated on collagen I were largely similar to cells on fibronectin after completed differentiation. Collectively, the data suggest that collagen I induces a more rapid and consistent differentiation of stem cells to definitive endoderm. The results shed light on the importance of extracellular matrix proteins for differentiation and also points to a cost effective and easy method to improve differentiation.

AB - Human embryonic stem cells have the ability to generate all cell types in the body and can potentially provide an unlimited source of cells for cell replacement therapy to treat degenerative diseases such as diabetes. Current differentiation protocols of human embryonic stem cells towards insulin producing beta cells focus on soluble molecules whereas the impact of cell-matrix interactions has been mainly unattended. In this study almost 500 different extracellular matrix protein combinations were screened to systemically identify extracellular matrix proteins that influence differentiation of human embryonic stem cells to the definitive endoderm lineage. The percentage of definitive endoderm cells after differentiation on collagen I and fibronectin was >85% and 65%, respectively. The cells on collagen I substrates displayed different morphology and gene expression during differentiation as assessed by time lapse studies compared to cells on the other tested substrates. Global gene expression analysis showed that cells differentiated on collagen I were largely similar to cells on fibronectin after completed differentiation. Collectively, the data suggest that collagen I induces a more rapid and consistent differentiation of stem cells to definitive endoderm. The results shed light on the importance of extracellular matrix proteins for differentiation and also points to a cost effective and easy method to improve differentiation.

U2 - 10.1371/journal.pone.0145389

DO - 10.1371/journal.pone.0145389

M3 - Journal article

C2 - 26713616

VL - 10

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 12

M1 - e0145389

ER -