Collaborative study - Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS

Petra Ložnjak Švarc*, Eystein Oveland, Hanna Sara Strandler, Susanna Kariluoto, Esther Campos-Giménez, Elise Ivarsen, Isabelle Malaviole, Carla Motta, Michael Rychlik, Lisa Striegel, Jette Jakobsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Quantification of the specific folate vitamers to estimate total folate in foods is not standardized. A collaborative study, including eight European laboratories, was conducted in order to determine the repeatability and reproducibility of the method for folate quantification in foods using the plant-origin γ-glutamyl hydrolase as part of the extraction procedure. The seven food samples analyzed represent the food groups; fruits, vegetables, dairy products, legumes, offal, fish, and fortified infant formula. The homogenization step was included, and six folate vitamers were analyzed using LC-MS/MS. Total folate content, expressed as folic acid equivalent, was 17–490 μg/100 g in all samples. Horwitz ratio values were within the acceptable range (0.60–1.94), except for fish. The results for fortified infant formula, a certified reference material (NIST 1869), confirmed the trueness of the method. The collaborative study is part of a standardization project within the Nordic Committee on Food Analysis (NMKL).

Original languageEnglish
Article number127447
JournalFood Chemistry
Number of pages10
Publication statusPublished - 2020


  • 10-Formylfolic acid (PubChem CID: 135405023)
  • 5,10-Methenyltetrahydrofolate (PubChem CID: 135398657)
  • 5-Formyltetrahydrofolate (PubChem CID: 135402009)
  • 5-Methyltetrahydrofolate (PubChem CID: 135415868)
  • Deconjugase
  • Folic acid (PubChem CID: 135398658)
  • Homogenization
  • Inter-laboratory study
  • Plant enzyme
  • Tetrahydrofolate (PubChem CID: 135444742)
  • Tetrahydrofolates


Dive into the research topics of 'Collaborative study - Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS'. Together they form a unique fingerprint.

Cite this