Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017)

Virginia Carfora; Patricia Alba; Pimlapas Leekitcharoenphon; Daniele Ballaro; Gessica Cordaro; Paola Di Matteo; Valentina  Donati; Angela Ianzano; Manuela Iurescia; Fiorentino Stravino; Tania Tagliaferri; Antonio Battisti; Alessia Franco

doi:10.3389/fmicb.2018.01880

Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017)

Virginia Carfora, Patricia Alba, Pimlapas Leekitcharoenphon, Daniele Ballaro, Gessica Cordaro, Paola Di Matteo, Valentina Donati, Angela Ianzano, Manuela Iurescia, Fiorentino Stravino, Tania Tagliaferri, Antonio Battisti^*, Alessia Franco

^*Corresponding author for this work

Experimental Zooprophylactic Institute of Lazio and Toscana

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Abstract

Colistin-resistance mediated by mobilisable and plasmid-borne mcr genes has emerged worldwide, threatening the efficacy of colistin, a last resort antibiotic increasingly used for treating human invasive infections by multidrug-resistant or extensively drug-resistant Enterobacteriaceae. In this study, we report the first evidence of mcr-1-mediated colistin resistance in four multidrug resistant (MDR) out of 324 Salmonella infantis from the Italian antimicrobial resistance (AMR) monitoring (2001-2017) in broilers and broiler meat. Two were also Extended Spectrum Beta-Lactamases (ESBL)-producing isolates. Characterization by whole genome sequencing (WGS), located mcr-1.1 on an incX4 plasmid. Phylogenetic analysis of these isolates with selected Italian S. Infantis previously isolated from animals, meat and human clinical cases with unknown epidemiological relationship, demonstrated that ESBL-producing, mcr-1-positive isolates belonged to the emerging pESI-like-positive-ESBL-producing clone described in Italy in 2015.

Original language	English
Article number	1880
Journal	Frontiers in Microbiology
Volume	9
Number of pages	6
ISSN	1664-302X
DOIs	https://doi.org/10.3389/fmicb.2018.01880
Publication status	Published - 2018

Keywords

colistin resistance
mcr genes
ESBL (Extended Spectrum Beta-Lactamases)
plasmids
whole genome sequencing
Salmonella Infantis
broilers
broiler meat
Microbiology
Microbiology (medical)
Broiler meat
Broilers
Colistin resistance
Mcr genes
Plasmids
Whole genome sequencing

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Carfora, V., Alba, P., Leekitcharoenphon, P., Ballaro, D., Cordaro, G., Di Matteo, P., Donati, V., Ianzano, A., Iurescia, M., Stravino, F., Tagliaferri, T., Battisti, A., & Franco, A. (2018). Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017). Frontiers in Microbiology, 9, Article 1880. https://doi.org/10.3389/fmicb.2018.01880

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title = "Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017)",

abstract = "Colistin-resistance mediated by mobilisable and plasmid-borne mcr genes has emerged worldwide, threatening the efficacy of colistin, a last resort antibiotic increasingly used for treating human invasive infections by multidrug-resistant or extensively drug-resistant Enterobacteriaceae. In this study, we report the first evidence of mcr-1-mediated colistin resistance in four multidrug resistant (MDR) out of 324 Salmonella infantis from the Italian antimicrobial resistance (AMR) monitoring (2001-2017) in broilers and broiler meat. Two were also Extended Spectrum Beta-Lactamases (ESBL)-producing isolates. Characterization by whole genome sequencing (WGS), located mcr-1.1 on an incX4 plasmid. Phylogenetic analysis of these isolates with selected Italian S. Infantis previously isolated from animals, meat and human clinical cases with unknown epidemiological relationship, demonstrated that ESBL-producing, mcr-1-positive isolates belonged to the emerging pESI-like-positive-ESBL-producing clone described in Italy in 2015.",

keywords = "colistin resistance, mcr genes, ESBL (Extended Spectrum Beta-Lactamases), plasmids, whole genome sequencing, Salmonella Infantis, broilers, broiler meat, Microbiology, Microbiology (medical), Broiler meat, Broilers, Colistin resistance, Mcr genes, Plasmids, Whole genome sequencing",

author = "Virginia Carfora and Patricia Alba and Pimlapas Leekitcharoenphon and Daniele Ballaro and Gessica Cordaro and {Di Matteo}, Paola and Valentina Donati and Angela Ianzano and Manuela Iurescia and Fiorentino Stravino and Tania Tagliaferri and Antonio Battisti and Alessia Franco",

year = "2018",

doi = "10.3389/fmicb.2018.01880",

language = "English",

volume = "9",

journal = "Frontiers in Microbiology",

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Carfora, V, Alba, P, Leekitcharoenphon, P, Ballaro, D, Cordaro, G, Di Matteo, P, Donati, V, Ianzano, A, Iurescia, M, Stravino, F, Tagliaferri, T, Battisti, A & Franco, A 2018, 'Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017)', Frontiers in Microbiology, vol. 9, 1880. https://doi.org/10.3389/fmicb.2018.01880

TY - JOUR

T1 - Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017)

AU - Carfora, Virginia

AU - Alba, Patricia

AU - Leekitcharoenphon, Pimlapas

AU - Ballaro, Daniele

AU - Cordaro, Gessica

AU - Di Matteo, Paola

AU - Donati, Valentina

AU - Ianzano, Angela

AU - Iurescia, Manuela

AU - Stravino, Fiorentino

AU - Tagliaferri, Tania

AU - Battisti, Antonio

AU - Franco, Alessia

PY - 2018

Y1 - 2018

N2 - Colistin-resistance mediated by mobilisable and plasmid-borne mcr genes has emerged worldwide, threatening the efficacy of colistin, a last resort antibiotic increasingly used for treating human invasive infections by multidrug-resistant or extensively drug-resistant Enterobacteriaceae. In this study, we report the first evidence of mcr-1-mediated colistin resistance in four multidrug resistant (MDR) out of 324 Salmonella infantis from the Italian antimicrobial resistance (AMR) monitoring (2001-2017) in broilers and broiler meat. Two were also Extended Spectrum Beta-Lactamases (ESBL)-producing isolates. Characterization by whole genome sequencing (WGS), located mcr-1.1 on an incX4 plasmid. Phylogenetic analysis of these isolates with selected Italian S. Infantis previously isolated from animals, meat and human clinical cases with unknown epidemiological relationship, demonstrated that ESBL-producing, mcr-1-positive isolates belonged to the emerging pESI-like-positive-ESBL-producing clone described in Italy in 2015.

AB - Colistin-resistance mediated by mobilisable and plasmid-borne mcr genes has emerged worldwide, threatening the efficacy of colistin, a last resort antibiotic increasingly used for treating human invasive infections by multidrug-resistant or extensively drug-resistant Enterobacteriaceae. In this study, we report the first evidence of mcr-1-mediated colistin resistance in four multidrug resistant (MDR) out of 324 Salmonella infantis from the Italian antimicrobial resistance (AMR) monitoring (2001-2017) in broilers and broiler meat. Two were also Extended Spectrum Beta-Lactamases (ESBL)-producing isolates. Characterization by whole genome sequencing (WGS), located mcr-1.1 on an incX4 plasmid. Phylogenetic analysis of these isolates with selected Italian S. Infantis previously isolated from animals, meat and human clinical cases with unknown epidemiological relationship, demonstrated that ESBL-producing, mcr-1-positive isolates belonged to the emerging pESI-like-positive-ESBL-producing clone described in Italy in 2015.

KW - colistin resistance

KW - mcr genes

KW - ESBL (Extended Spectrum Beta-Lactamases)

KW - plasmids

KW - whole genome sequencing

KW - Salmonella Infantis

KW - broilers

KW - broiler meat

KW - Microbiology

KW - Microbiology (medical)

KW - Broiler meat

KW - Broilers

KW - Colistin resistance

KW - Mcr genes

KW - Plasmids

KW - Whole genome sequencing

U2 - 10.3389/fmicb.2018.01880

DO - 10.3389/fmicb.2018.01880

M3 - Journal article

C2 - 30174660

SN - 1664-302X

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 1880

ER -

Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017)

Abstract

Keywords

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