@inbook{e9669a5fba664ea4b973696f7350199f,
title = "Codon optimizing for increased membrane protein production: a minimalist approach",
abstract = "Reengineering a gene with synonymous codons is a popular approach for increasing production levels of recombinant proteins. Here we present a minimalist alternative to this method, which samples synonymous codons only at the second and third positions rather than the entire coding sequence. As demonstrated with two membrane-embedded transporters in Escherichia coli, the method was more effective than optimizing the entire coding sequence. The method we present is PCR based and requires three simple steps: (1) the design of two PCR primers, one of which is degenerate; (2) the amplification of a minilibrary by PCR; and (3) screening for high-expressing clones.",
keywords = "Membrane protein, Protein expression, Codon optimization, Synonymous codon",
author = "K. Mirzadeh and S. Toddo and Morten N{\o}rholm and Daley, \{Daniel O.\}",
year = "2016",
doi = "10.1007/978-1-4939-3637-3\_4",
language = "English",
isbn = "978-1-4939-3635-9",
volume = "1432",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "53--61",
editor = "Isabelle Mus-Veteau",
booktitle = "Heterologous Expression of Membrane Proteins",
}