Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells

Lauri J. Reuter; Mohammad-Ali Shahbazi; Ermei M. Makila; Jamo J. Salonen; Reza Saberianfar; Rima Menassa; Hélder A. Santos; Jussi J. Joensuu; Anneli Ritala

doi:10.1021/acs.bioconjchem.7b00075

Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells

Lauri J. Reuter, Mohammad-Ali Shahbazi, Ermei M. Makila, Jamo J. Salonen, Reza Saberianfar, Rima Menassa, Hélder A. Santos, Jussi J. Joensuu, Anneli Ritala

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The encapsulation of drugs to nanoparticles may offer a solution for targeted delivery. Here, we set out to engineer a self assembling targeting ligand by combining the functional properties of human transferrin and fungal hydrophobins in a single fusion protein. We showed that human transferrin can be expressed in Nicotiana benthamiana plants as a fusion with Trichoderma reesei hydrophobins HFBI, HFBII, or HFBIV. Transferrin-HFBIV was further expressed in tobacco BY-2 suspension cells. Both partners of the fusion protein retained their functionality; the hydrophobin moiety enabled migration to a surfactant phase in an aqueous two-phase system, and the transferrin moiety was able to reversibly bind iron. Coating porous silicon nanoparticles with the fusion protein resulted in uptake of the nanoparticles in human cancer cells. This study provides a proof-of concept for the functionalization of hydrophobin coatings with transferrin as a targeting ligand.

Original language	English
Journal	Bioconjugate Chemistry
Volume	28
Issue number	6
Pages (from-to)	1639-1648
Number of pages	10
ISSN	1043-1802
DOIs	https://doi.org/10.1021/acs.bioconjchem.7b00075
Publication status	Published - 2017

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title = "Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells",

abstract = "The encapsulation of drugs to nanoparticles may offer a solution for targeted delivery. Here, we set out to engineer a self assembling targeting ligand by combining the functional properties of human transferrin and fungal hydrophobins in a single fusion protein. We showed that human transferrin can be expressed in Nicotiana benthamiana plants as a fusion with Trichoderma reesei hydrophobins HFBI, HFBII, or HFBIV. Transferrin-HFBIV was further expressed in tobacco BY-2 suspension cells. Both partners of the fusion protein retained their functionality; the hydrophobin moiety enabled migration to a surfactant phase in an aqueous two-phase system, and the transferrin moiety was able to reversibly bind iron. Coating porous silicon nanoparticles with the fusion protein resulted in uptake of the nanoparticles in human cancer cells. This study provides a proof-of concept for the functionalization of hydrophobin coatings with transferrin as a targeting ligand.",

author = "Reuter, {Lauri J.} and Mohammad-Ali Shahbazi and Makila, {Ermei M.} and Salonen, {Jamo J.} and Reza Saberianfar and Rima Menassa and Santos, {H{\'e}lder A.} and Joensuu, {Jussi J.} and Anneli Ritala",

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doi = "10.1021/acs.bioconjchem.7b00075",

language = "English",

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T1 - Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells

AU - Reuter, Lauri J.

AU - Shahbazi, Mohammad-Ali

AU - Makila, Ermei M.

AU - Salonen, Jamo J.

AU - Saberianfar, Reza

AU - Menassa, Rima

AU - Santos, Hélder A.

AU - Joensuu, Jussi J.

AU - Ritala, Anneli

PY - 2017

Y1 - 2017

N2 - The encapsulation of drugs to nanoparticles may offer a solution for targeted delivery. Here, we set out to engineer a self assembling targeting ligand by combining the functional properties of human transferrin and fungal hydrophobins in a single fusion protein. We showed that human transferrin can be expressed in Nicotiana benthamiana plants as a fusion with Trichoderma reesei hydrophobins HFBI, HFBII, or HFBIV. Transferrin-HFBIV was further expressed in tobacco BY-2 suspension cells. Both partners of the fusion protein retained their functionality; the hydrophobin moiety enabled migration to a surfactant phase in an aqueous two-phase system, and the transferrin moiety was able to reversibly bind iron. Coating porous silicon nanoparticles with the fusion protein resulted in uptake of the nanoparticles in human cancer cells. This study provides a proof-of concept for the functionalization of hydrophobin coatings with transferrin as a targeting ligand.

AB - The encapsulation of drugs to nanoparticles may offer a solution for targeted delivery. Here, we set out to engineer a self assembling targeting ligand by combining the functional properties of human transferrin and fungal hydrophobins in a single fusion protein. We showed that human transferrin can be expressed in Nicotiana benthamiana plants as a fusion with Trichoderma reesei hydrophobins HFBI, HFBII, or HFBIV. Transferrin-HFBIV was further expressed in tobacco BY-2 suspension cells. Both partners of the fusion protein retained their functionality; the hydrophobin moiety enabled migration to a surfactant phase in an aqueous two-phase system, and the transferrin moiety was able to reversibly bind iron. Coating porous silicon nanoparticles with the fusion protein resulted in uptake of the nanoparticles in human cancer cells. This study provides a proof-of concept for the functionalization of hydrophobin coatings with transferrin as a targeting ligand.

U2 - 10.1021/acs.bioconjchem.7b00075

DO - 10.1021/acs.bioconjchem.7b00075

M3 - Journal article

C2 - 28557453

SN - 1043-1802

VL - 28

SP - 1639

EP - 1648

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 6

ER -

Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells

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