Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid

Jakub Zdarta*, Karolina Bachosz, Oliwia Degórska, Agata Zdarta, Ewa Kaczorek, Manuel Pinelo, Anne S. Meyer, Teofil Jesionowski

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The conversion of biomass components catalyzed via immobilized enzymes is a promising way of obtaining valuable compounds with high efficiency under mild conditions. However, simultaneous transformation of glucose and xylose into gluconic acid and xylonic acid, respectively, is an overlooked research area. Therefore, in this work we have undertaken a study focused on the co-immobilization of glucose dehydrogenase (GDH, EC 1.1.1.118) and xylose dehydrogenase (XDH, EC 1.1.1.175) using mesoporous Santa Barbara Amorphous silica (SBA 15) for the simultaneous production of gluconic acid and xylonic acid. The effective co-immobilization of enzymes onto the surface and into the pores of the silica support was confirmed. A GDH:XDH ratio equal to 1:5 was the most suitable for the conversion of xylose and glucose, as the reaction yield reached over 90% for both monosaccharides after 45 min of the process. Upon co-immobilization, reaction yields exceeding 80% were noticed over wide pH (7-9) and temperature (40-60 °C) ranges. Additionally, the co-immobilized GDH and XDH exhibited a significant enhancement of their thermal, chemical and storage stability. Furthermore, the co-immobilized enzymes are characterized by good reusability, as they facilitated the reaction yields by over 80%, even after 5 consecutive reaction steps.
Original languageEnglish
Article number3167
JournalMaterials
Volume12
Issue number19
Number of pages16
ISSN1996-1944
DOIs
Publication statusPublished - 2019

Keywords

  • Glucose dehydrogenase
  • Xylose dehydrogenase
  • Enzymes immobilization
  • Co-immobilization
  • Silica SBA 15
  • Biomass conversion

Cite this

Zdarta, Jakub ; Bachosz, Karolina ; Degórska, Oliwia ; Zdarta, Agata ; Kaczorek, Ewa ; Pinelo, Manuel ; Meyer, Anne S. ; Jesionowski, Teofil. / Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid. In: Materials. 2019 ; Vol. 12, No. 19.
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title = "Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid",
abstract = "The conversion of biomass components catalyzed via immobilized enzymes is a promising way of obtaining valuable compounds with high efficiency under mild conditions. However, simultaneous transformation of glucose and xylose into gluconic acid and xylonic acid, respectively, is an overlooked research area. Therefore, in this work we have undertaken a study focused on the co-immobilization of glucose dehydrogenase (GDH, EC 1.1.1.118) and xylose dehydrogenase (XDH, EC 1.1.1.175) using mesoporous Santa Barbara Amorphous silica (SBA 15) for the simultaneous production of gluconic acid and xylonic acid. The effective co-immobilization of enzymes onto the surface and into the pores of the silica support was confirmed. A GDH:XDH ratio equal to 1:5 was the most suitable for the conversion of xylose and glucose, as the reaction yield reached over 90{\%} for both monosaccharides after 45 min of the process. Upon co-immobilization, reaction yields exceeding 80{\%} were noticed over wide pH (7-9) and temperature (40-60 °C) ranges. Additionally, the co-immobilized GDH and XDH exhibited a significant enhancement of their thermal, chemical and storage stability. Furthermore, the co-immobilized enzymes are characterized by good reusability, as they facilitated the reaction yields by over 80{\%}, even after 5 consecutive reaction steps.",
keywords = "Glucose dehydrogenase, Xylose dehydrogenase, Enzymes immobilization, Co-immobilization, Silica SBA 15, Biomass conversion",
author = "Jakub Zdarta and Karolina Bachosz and Oliwia Deg{\'o}rska and Agata Zdarta and Ewa Kaczorek and Manuel Pinelo and Meyer, {Anne S.} and Teofil Jesionowski",
year = "2019",
doi = "10.3390/ma12193167",
language = "English",
volume = "12",
journal = "Materials",
issn = "1996-1944",
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Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid. / Zdarta, Jakub; Bachosz, Karolina; Degórska, Oliwia; Zdarta, Agata; Kaczorek, Ewa; Pinelo, Manuel; Meyer, Anne S.; Jesionowski, Teofil.

In: Materials, Vol. 12, No. 19, 3167, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid

AU - Zdarta, Jakub

AU - Bachosz, Karolina

AU - Degórska, Oliwia

AU - Zdarta, Agata

AU - Kaczorek, Ewa

AU - Pinelo, Manuel

AU - Meyer, Anne S.

AU - Jesionowski, Teofil

PY - 2019

Y1 - 2019

N2 - The conversion of biomass components catalyzed via immobilized enzymes is a promising way of obtaining valuable compounds with high efficiency under mild conditions. However, simultaneous transformation of glucose and xylose into gluconic acid and xylonic acid, respectively, is an overlooked research area. Therefore, in this work we have undertaken a study focused on the co-immobilization of glucose dehydrogenase (GDH, EC 1.1.1.118) and xylose dehydrogenase (XDH, EC 1.1.1.175) using mesoporous Santa Barbara Amorphous silica (SBA 15) for the simultaneous production of gluconic acid and xylonic acid. The effective co-immobilization of enzymes onto the surface and into the pores of the silica support was confirmed. A GDH:XDH ratio equal to 1:5 was the most suitable for the conversion of xylose and glucose, as the reaction yield reached over 90% for both monosaccharides after 45 min of the process. Upon co-immobilization, reaction yields exceeding 80% were noticed over wide pH (7-9) and temperature (40-60 °C) ranges. Additionally, the co-immobilized GDH and XDH exhibited a significant enhancement of their thermal, chemical and storage stability. Furthermore, the co-immobilized enzymes are characterized by good reusability, as they facilitated the reaction yields by over 80%, even after 5 consecutive reaction steps.

AB - The conversion of biomass components catalyzed via immobilized enzymes is a promising way of obtaining valuable compounds with high efficiency under mild conditions. However, simultaneous transformation of glucose and xylose into gluconic acid and xylonic acid, respectively, is an overlooked research area. Therefore, in this work we have undertaken a study focused on the co-immobilization of glucose dehydrogenase (GDH, EC 1.1.1.118) and xylose dehydrogenase (XDH, EC 1.1.1.175) using mesoporous Santa Barbara Amorphous silica (SBA 15) for the simultaneous production of gluconic acid and xylonic acid. The effective co-immobilization of enzymes onto the surface and into the pores of the silica support was confirmed. A GDH:XDH ratio equal to 1:5 was the most suitable for the conversion of xylose and glucose, as the reaction yield reached over 90% for both monosaccharides after 45 min of the process. Upon co-immobilization, reaction yields exceeding 80% were noticed over wide pH (7-9) and temperature (40-60 °C) ranges. Additionally, the co-immobilized GDH and XDH exhibited a significant enhancement of their thermal, chemical and storage stability. Furthermore, the co-immobilized enzymes are characterized by good reusability, as they facilitated the reaction yields by over 80%, even after 5 consecutive reaction steps.

KW - Glucose dehydrogenase

KW - Xylose dehydrogenase

KW - Enzymes immobilization

KW - Co-immobilization

KW - Silica SBA 15

KW - Biomass conversion

U2 - 10.3390/ma12193167

DO - 10.3390/ma12193167

M3 - Journal article

VL - 12

JO - Materials

JF - Materials

SN - 1996-1944

IS - 19

M1 - 3167

ER -