TY - JOUR
T1 - Co-delivery of ciprofloxacin and colistin using microcontainers for bacterial biofilm treatment
AU - Birk, Stine Egebro
AU - Mazzoni, Chiara
AU - Mobasharah Javed, Madeeha
AU - Borre Hansen, Morten
AU - Krogh Johansen, Helle
AU - Anders Juul Haagensen, Janus
AU - Molin, Søren
AU - Hagner Nielsen, Line
AU - Boisen, Anja
PY - 2021
Y1 - 2021
N2 - In many infected patients, bacterial biofilms represent a mode of growth that significantly enhances the tolerance to antimicrobials, leaving the patients with difficult-to-cure infections. Therefore, there is a growing need for effective treatment strategies to combat biofilm infections. In this work, reservoir-based microdevices, also known as microcontainers (MCs), are co-loaded with two antibiotics: ciprofloxacin hydrochloride (CIP) and colistin sulfate (COL), targeting both metabolically active and dormant subpopulations of the biofilm. We assess the effect of the two drugs in a time-kill study of planktonic P. aeruginosa and find that co-loaded MCs are superior to monotherapy, resulting in complete killing of the entire population. Biofilm consortia of P. aeruginosa grown in flow chambers were not fully eradicated. However, antibiotics in MCs work significantly faster than simple perfusion of antibiotics (62.5 ± 8.3% versus 10.6 ± 10.1% after 5 h) in biofilm consortia, showing the potential of the MC-based treatment to minimize the use of antimicrobials in future therapies.
AB - In many infected patients, bacterial biofilms represent a mode of growth that significantly enhances the tolerance to antimicrobials, leaving the patients with difficult-to-cure infections. Therefore, there is a growing need for effective treatment strategies to combat biofilm infections. In this work, reservoir-based microdevices, also known as microcontainers (MCs), are co-loaded with two antibiotics: ciprofloxacin hydrochloride (CIP) and colistin sulfate (COL), targeting both metabolically active and dormant subpopulations of the biofilm. We assess the effect of the two drugs in a time-kill study of planktonic P. aeruginosa and find that co-loaded MCs are superior to monotherapy, resulting in complete killing of the entire population. Biofilm consortia of P. aeruginosa grown in flow chambers were not fully eradicated. However, antibiotics in MCs work significantly faster than simple perfusion of antibiotics (62.5 ± 8.3% versus 10.6 ± 10.1% after 5 h) in biofilm consortia, showing the potential of the MC-based treatment to minimize the use of antimicrobials in future therapies.
U2 - 10.1016/j.ijpharm.2021.120420
DO - 10.1016/j.ijpharm.2021.120420
M3 - Journal article
C2 - 33647404
SN - 0378-5173
VL - 599
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
M1 - 120420
ER -