CLUSEAN: a computer-based framework for the automated analysis of bacterial secondary metabolite biosynthetic gene clusters: Journal of Biotechnology

Tilmann Weber, C. Rausch, P. Lopez, I. Hoof, V. Gaykova, D. H. Huson, W. Wohlleben

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Bacterial secondary metabolites are an important source of antimicrobial and cytostatic drugs. These molecules are often synthesized in a stepwise fashion by multimodular megaenzymes that are encoded in clusters of genes encoding enzymes for precursor supply and modification. In this work,we present an open source software pipeline, CLUSEAN (CLUster SEquence ANalyzer) that helps to annotate and analyze such gene clusters. CLUSEAN integrates standard analysis tools, like BLAST and HMMer, with specific tools for the identification of the functional domains and motifs in nonribosomal peptide synthetases (NRPS)/type I polyketide synthases (PKS) and the prediction of specificities of NRPS.
Original languageEnglish
JournalJournal of biotechnology
Volume140
Pages (from-to)13-17
Number of pages5
ISSN1873-4863
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Anti-Bacterial Agents/metabolism Bacteria/*genetics/metabolism Bacterial Proteins/chemistry/genetics/metabolism Computer Simulation Databases, Genetic *Genes, Bacterial Macrolides/*metabolism *Multigene Family Peptide Biosynthesis, Nucleic Acid-Independent *Polyketide Synthases/chemistry/genetics/metabolism Protein Structure, Tertiary/genetics *Software

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