Cloning, sequencing and expression of a xylanase gene from the maize pathogen Helminthosporium turcicum

Y. Degefu, L. Paulin, Peter Stephensen Lübeck

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A gene encoding an endoxylanase from the phytopathogenic fungus Helminthosporium turcicum Pass. was cloned and sequenced. The entire nucleotide sequence of a 1991 bp genomic fragment containing an endoxylanase gene was determined. The xylanase gene of 795 bp, interrupted by two introns of 52 and 62 bp, encoded a protein of 227 amino acids showing up to 95% amino acid homology with other fungal xylanases. The precise splicing site of the introns was identified by sequencing the corresponding cDNA. A northern blot showed that the gene is expressed when the fungus is grown in a medium containing xylan as a sole carbon source. The cloned xylanase gene was expressed in maize plants during infection.
    Original languageEnglish
    JournalEuropean Journal of Plant Pathology
    Volume107
    Issue number4
    Pages (from-to)457-465
    ISSN0929-1873
    DOIs
    Publication statusPublished - 2001

    Keywords

    • Zea mays
    • Endoxylanase
    • Helminthosporium turcicum

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