Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola

Rustam Aminov; T. Nagamine; K. Ogata; M. Sugiura; K. Tajima; Y. Benno

doi:10.1016/0378-1097(96)00338-2

Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola

Rustam Aminov
, T. Nagamine
, K. Ogata
, M. Sugiura
, K. Tajima
, Y. Benno

STAFF-Institute

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recE from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa. P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coil recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen.

Original language	English
Journal	F E M S Microbiology Letters
Volume	144
Issue number	1
Pages (from-to)	53-59
Number of pages	7
ISSN	0378-1097
DOIs	https://doi.org/10.1016/0378-1097(96)00338-2
Publication status	Published - 1996
Externally published	Yes

Keywords

Amino Acid Sequence
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Genetic Complementation Test
Methyl Methanesulfonate
Mitomycin
Molecular Sequence Data
Mutagens
Prevotella
Rec A Recombinases
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Ultraviolet Rays
50SG953SK6 Mitomycin
AT5C31J09G Methyl Methanesulfonate
EC 2.7.7.- Rec A Recombinases
article
controlled study
dna sequence
escherichia coli
genetic analysis
genetic complementation
molecular cloning
nonhuman
polymerase chain reaction
prevotella
priority journal
prophage
Bacteroides fragilis
Prevotella ruminicola
Prevotella ruminicola 23
complementation
RecA
MICROBIOLOGY
RECOMBINATION
BACTERIA
PROTEIN
Complementation
CLONING
COMPLEMENTATION ANALYSIS
METHYL METHANE SULFONATE
MITOMYCIN C
MOLECULAR GENETICS
PROPHAGE INDUCTION
RECA GENE
RECA PROTEIN
SENSITIVITY
SEQUENCING
STRAIN-23
Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Prevotella ruminicola
Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli
METHYL METHANE SULFONATE 66-27-3
MITOMYCIN C 50-07-7
10064, Biochemistry studies - Proteins, peptides and amino acids
10300, Replication, transcription, translation
10506, Biophysics - Molecular properties and macromolecules
13012, Metabolism - Proteins, peptides and amino acids
13014, Metabolism - Nucleic acids, purines and pyrimidines
31000, Physiology and biochemistry of bacteria
31500, Genetics of bacteria and viruses
33504, Virology - Bacteriophage
Biochemistry and Molecular Biophysics
Genetics
Metabolism
Microbiology
Molecular Genetics
Physiology

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title = "Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola",

abstract = "Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recE from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa. P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75\% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coil recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen.",

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author = "Rustam Aminov and T. Nagamine and K. Ogata and M. Sugiura and K. Tajima and Y. Benno",

year = "1996",

doi = "10.1016/0378-1097(96)00338-2",

language = "English",

volume = "144",

pages = "53--59",

journal = "F E M S Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola

AU - Aminov, Rustam

AU - Nagamine, T.

AU - Ogata, K.

AU - Sugiura, M.

AU - Tajima, K.

AU - Benno, Y.

PY - 1996

Y1 - 1996

N2 - Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recE from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa. P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coil recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen.

AB - Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recE from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa. P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coil recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen.

KW - Amino Acid Sequence

KW - Cloning, Molecular

KW - Escherichia coli

KW - Genes, Bacterial

KW - Genetic Complementation Test

KW - Methyl Methanesulfonate

KW - Mitomycin

KW - Molecular Sequence Data

KW - Mutagens

KW - Prevotella

KW - Rec A Recombinases

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Ultraviolet Rays

KW - 50SG953SK6 Mitomycin

KW - AT5C31J09G Methyl Methanesulfonate

KW - EC 2.7.7.- Rec A Recombinases

KW - article

KW - controlled study

KW - dna sequence

KW - escherichia coli

KW - genetic analysis

KW - genetic complementation

KW - molecular cloning

KW - nonhuman

KW - polymerase chain reaction

KW - prevotella

KW - priority journal

KW - prophage

KW - Bacteroides fragilis

KW - Prevotella ruminicola

KW - Prevotella ruminicola 23

KW - complementation

KW - RecA

KW - MICROBIOLOGY

KW - RECOMBINATION

KW - BACTERIA

KW - PROTEIN

KW - Complementation

KW - CLONING

KW - COMPLEMENTATION ANALYSIS

KW - METHYL METHANE SULFONATE

KW - MITOMYCIN C

KW - MOLECULAR GENETICS

KW - PROPHAGE INDUCTION

KW - RECA GENE

KW - RECA PROTEIN

KW - SENSITIVITY

KW - SEQUENCING

KW - STRAIN-23

KW - Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Prevotella ruminicola

KW - Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli

KW - METHYL METHANE SULFONATE 66-27-3

KW - MITOMYCIN C 50-07-7

KW - 10064, Biochemistry studies - Proteins, peptides and amino acids

KW - 10300, Replication, transcription, translation

KW - 10506, Biophysics - Molecular properties and macromolecules

KW - 13012, Metabolism - Proteins, peptides and amino acids

KW - 13014, Metabolism - Nucleic acids, purines and pyrimidines

KW - 31000, Physiology and biochemistry of bacteria

KW - 31500, Genetics of bacteria and viruses

KW - 33504, Virology - Bacteriophage

KW - Biochemistry and Molecular Biophysics

KW - Genetics

KW - Metabolism

KW - Microbiology

KW - Molecular Genetics

KW - Physiology

U2 - 10.1016/0378-1097(96)00338-2

DO - 10.1016/0378-1097(96)00338-2

M3 - Journal article

SN - 0378-1097

VL - 144

SP - 53

EP - 59

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

IS - 1

ER -

Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola

Abstract

Keywords

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