Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola

Rustam Aminov, T. Nagamine, K. Ogata, M. Sugiura, K. Tajima, Y. Benno

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Abstract

Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recE from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa. P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coil recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen.
Original languageEnglish
JournalF E M S Microbiology Letters
Volume144
Issue number1
Pages (from-to)53-59
Number of pages7
ISSN0378-1097
DOIs
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • Amino Acid Sequence
  • Cloning, Molecular
  • Escherichia coli
  • Genes, Bacterial
  • Genetic Complementation Test
  • Methyl Methanesulfonate
  • Mitomycin
  • Molecular Sequence Data
  • Mutagens
  • Prevotella
  • Rec A Recombinases
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Ultraviolet Rays
  • 50SG953SK6 Mitomycin
  • AT5C31J09G Methyl Methanesulfonate
  • EC 2.7.7.- Rec A Recombinases
  • article
  • controlled study
  • dna sequence
  • escherichia coli
  • genetic analysis
  • genetic complementation
  • molecular cloning
  • nonhuman
  • polymerase chain reaction
  • prevotella
  • priority journal
  • prophage
  • Bacteroides fragilis
  • Prevotella ruminicola
  • Prevotella ruminicola 23
  • complementation
  • RecA
  • MICROBIOLOGY
  • RECOMBINATION
  • BACTERIA
  • PROTEIN
  • Complementation
  • CLONING
  • COMPLEMENTATION ANALYSIS
  • METHYL METHANE SULFONATE
  • MITOMYCIN C
  • MOLECULAR GENETICS
  • PROPHAGE INDUCTION
  • RECA GENE
  • RECA PROTEIN
  • SENSITIVITY
  • SEQUENCING
  • STRAIN-23
  • Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Prevotella ruminicola
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli
  • METHYL METHANE SULFONATE 66-27-3
  • MITOMYCIN C 50-07-7
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10300, Replication, transcription, translation
  • 10506, Biophysics - Molecular properties and macromolecules
  • 13012, Metabolism - Proteins, peptides and amino acids
  • 13014, Metabolism - Nucleic acids, purines and pyrimidines
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 33504, Virology - Bacteriophage
  • Biochemistry and Molecular Biophysics
  • Genetics
  • Metabolism
  • Microbiology
  • Molecular Genetics
  • Physiology

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