Cloning arbuscule-related genes from mycorrhizas

Stephen Burleigh

    Research output: Contribution to journalConference articleResearchpeer-review

    Abstract

    Until recently little was known about the identity of the genes expressed in the arbuscules of mycorrhizas, due in part to problems associated with cloning genes from the tissues of an obligate symbiont. However, the combination of advanced molecular techniques, innovative use of the materials available and fortuitous cloning has resulted in the recent identification of a number of arbuscule-related genes. This article provides a brief summary of the genes involved in arbuscule development, function and regulation, and the techniques used to study them. Molecular techniques include differential screening, differential display and screening with heterologous probes, and can involve the use of mycorrhizal plant mutants. New technologies such as proteome analysis are also discussed.
    Original languageEnglish
    JournalPlant and Soil
    Volume226
    Issue number2
    Pages (from-to)287-292
    ISSN0032-079X
    DOIs
    Publication statusPublished - 2000
    EventWorkshop on Methods for Study of the Hyphae of AMF - Uppsala, Sweden
    Duration: 1 Jul 19984 Jul 1998

    Workshop

    WorkshopWorkshop on Methods for Study of the Hyphae of AMF
    CountrySweden
    CityUppsala
    Period01/07/199804/07/1998

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