Clonality of Vibrio anguillarum strains isolated from fish from the Scandinavian countries, Sweden, Finland and Denmark

Karl Pedersen, I. Kühn, J. Seppanen, A. Hellstrom, T. Tiainen, E. Rimaila-Parnanen, J.L. Larsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In order to investigate whether outbreaks of vibriosis in the Baltic region were caused by the spread of certain pathogenic clones, 291 Vibrio anguillarum isolates from Finland (n = 156), Sweden (n = 88) and Denmark (n = 47) were studied with respect to serogroup, ribotype, plasmid content, and biochemical phenotypes as expressed with the PhenePlate (PhP) typing system. For comparison, 54 V. anguillarum serogroup O1 from other countries worldwide were included. Most isolates from Finland, Sweden and Denmark belonged to serogroup O1 (255), followed by O2 (30). Four Finnish isolates cross-reacted strongly with antisera against two new serogroups VaNT2 and VaNT4, whereas two strains were non-typeable. The serogroup O1 isolates displayed ten different ribotype patterns, whereas the other strains were considerably more diverse with respect to ribotypes. Most of the O1 isolates carried the 67 kb virulence plasmid and a group of Finnish isolates, in addition, carried an 86 kb plasmid. Additional plasmids with molecular weights of 63, 76, 135 or 260-290 kb were found in single O1 isolates. With few exceptions, strains of serogroup O2 either had no plasmids or carried one or two Small plasmids. PhenePlate typing revealed considerable diversity within the species, serogroup O1 being the most homogeneous. A few PhP types were dominant, whereas other types were observed only in one to four isolates. The prevalence of the different types changed significantly from one year to another but in Finland, one clonal lineage became increasingly important from 1992 (20% of isolates) to 1996 (80%). Remaining clones were mostly restricted to specific geographic areas. By cluster analysis, it was demonstrated that most of the isolates from Finland, Sweden and Denmark belonged to two clusters, and most of the strains from Southern Europe fell into two other, distinct clusters. Most isolates from the UK, North America, Chile and Tasmania grouped together in a distinct cluster. For the typing of V. anguillarum, O-serotyping should be the primary method. For isolates belonging to serogroups other than O1, plasmid profiling in combination with ribotyping gives a very good discrimination between strains, whereas for serogroup O1, another method is required. It is concluded that PhP typing is a tool that provides a good discrimination between O1 isolates.
Original languageEnglish
JournalJournal of Applied Microbiology
Volume86
Issue number2
Pages (from-to)337-347
Number of pages11
ISSN1364-5072
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • Animals
  • Bacterial Typing Techniques
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fish Diseases
  • Fishes
  • Genes, rRNA
  • Phylogeny
  • Plasmids
  • Scandinavia
  • Serotyping
  • Vibrio
  • Vibrio Infections
  • antiserum
  • article
  • Denmark
  • Finland
  • fish
  • nonhuman
  • phenotype
  • plasmid
  • ribotyping
  • serotyping
  • Sweden
  • vibrio
  • Listonella anguillarum
  • Negibacteria
  • BIOTECHNOLOGY
  • MICROBIOLOGY
  • IRON-TRANSPORT GENES
  • GEL-ELECTROPHORESIS
  • PLASMID
  • VIRULENCE
  • PROTEIN
  • O1
  • DIVERSITY
  • RIBOTYPES
  • PROFILES
  • SYSTEM
  • Chile South America Neotropical region
  • Denmark Europe Palearctic region
  • Finland Europe Palearctic region
  • North America Nearctic region
  • Sweden Europe Palearctic region
  • Tasmania Australia Australasian region
  • UK Europe Palearctic region
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio anguillarum Baltic isolates, VaNT2 serogroup, VaNT4 serogroup, serogroup O1, clonality, food contaminant, serogroup O2
  • Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] fish host
  • virulence plasmid
  • 00532, General biology - Miscellaneous
  • 10050, Biochemistry methods - General
  • 10060, Biochemistry studies - General
  • 13502, Food technology - General and methods
  • 30000, Bacteriology, general and systematic
  • 31500, Genetics of bacteria and viruses
  • 36001, Medical and clinical microbiology - General and methods
  • 37056, Public health: epidemiology - Miscellaneous
  • Epidemiology
  • Foods
  • Population Genetics
  • Vector Biology
  • vibriosis Vibrio Infections (MeSH) bacterial disease
  • vibriosis outbreak
  • Population Studies
  • PhenePlate typing system analytical method, genetic method

Cite this

Pedersen, K., Kühn, I., Seppanen, J., Hellstrom, A., Tiainen, T., Rimaila-Parnanen, E., & Larsen, J. L. (1999). Clonality of Vibrio anguillarum strains isolated from fish from the Scandinavian countries, Sweden, Finland and Denmark. Journal of Applied Microbiology, 86(2), 337-347. https://doi.org/10.1046/j.1365-2672.1999.00658.x