TY - JOUR
T1 - Clonal origin of aminoglycoside-resistant Citrobacter freundii isolates in a Danish county
AU - Norskov-Lauritsen, N.
AU - Sandvang, Dorthe
AU - Hedegaard, J.
AU - Fussing, V.
AU - Mortensen, K.K.
AU - Sperling-Petersen, H.U.
AU - Schonheyder, H.C.
PY - 2001
Y1 - 2001
N2 - During 1997, attention was drawn to an increased frequency of aminoglycoside-resistant Citrobacter freundii in a Danish county, when a total of 24 resistant C. freundii isolates was detected. In this study, 15 such isolates were typed by pulsed-field gel electrophoresis, riboprinting and partial sequencing of the gene encoding translation initiation factor 2. Fourteen of the 15 isolates were identical, as evaluated by their antibiograms and by all these typing methods, This epidemic strain harboured the aminoglycoside resistance genes aac(3)-II and ant(3")-I, with the latter located in tandem with a dihydrofolate reductase gene in a class I integron. The source of the strain remains unresolved. Representative isolates were obtained from various specimens from hospitals and general practice throughout the county, with no evidence of patient-to-patient transmission.
AB - During 1997, attention was drawn to an increased frequency of aminoglycoside-resistant Citrobacter freundii in a Danish county, when a total of 24 resistant C. freundii isolates was detected. In this study, 15 such isolates were typed by pulsed-field gel electrophoresis, riboprinting and partial sequencing of the gene encoding translation initiation factor 2. Fourteen of the 15 isolates were identical, as evaluated by their antibiograms and by all these typing methods, This epidemic strain harboured the aminoglycoside resistance genes aac(3)-II and ant(3")-I, with the latter located in tandem with a dihydrofolate reductase gene in a class I integron. The source of the strain remains unresolved. Representative isolates were obtained from various specimens from hospitals and general practice throughout the county, with no evidence of patient-to-patient transmission.
M3 - Journal article
SN - 0022-2615
VL - 50
SP - 636
EP - 641
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - 7
ER -