Clonal origin of aminoglycoside-resistant Citrobacter freundii isolates in a Danish county

N. Norskov-Lauritsen; Dorthe Sandvang; J. Hedegaard; V. Fussing; K.K. Mortensen; H.U. Sperling-Petersen; H.C. Schonheyder

Clonal origin of aminoglycoside-resistant Citrobacter freundii isolates in a Danish county

N. Norskov-Lauritsen, Dorthe Sandvang, J. Hedegaard, V. Fussing, K.K. Mortensen, H.U. Sperling-Petersen, H.C. Schonheyder

Technical University of Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

During 1997, attention was drawn to an increased frequency of aminoglycoside-resistant Citrobacter freundii in a Danish county, when a total of 24 resistant C. freundii isolates was detected. In this study, 15 such isolates were typed by pulsed-field gel electrophoresis, riboprinting and partial sequencing of the gene encoding translation initiation factor 2. Fourteen of the 15 isolates were identical, as evaluated by their antibiograms and by all these typing methods, This epidemic strain harboured the aminoglycoside resistance genes aac(3)-II and ant(3")-I, with the latter located in tandem with a dihydrofolate reductase gene in a class I integron. The source of the strain remains unresolved. Representative isolates were obtained from various specimens from hospitals and general practice throughout the county, with no evidence of patient-to-patient transmission.

Original language	English
Journal	Journal of Medical Microbiology
Volume	50
Issue number	7
Pages (from-to)	636-641
ISSN	0022-2615
Publication status	Published - 2001

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title = "Clonal origin of aminoglycoside-resistant Citrobacter freundii isolates in a Danish county",

abstract = "During 1997, attention was drawn to an increased frequency of aminoglycoside-resistant Citrobacter freundii in a Danish county, when a total of 24 resistant C. freundii isolates was detected. In this study, 15 such isolates were typed by pulsed-field gel electrophoresis, riboprinting and partial sequencing of the gene encoding translation initiation factor 2. Fourteen of the 15 isolates were identical, as evaluated by their antibiograms and by all these typing methods, This epidemic strain harboured the aminoglycoside resistance genes aac(3)-II and ant(3{"})-I, with the latter located in tandem with a dihydrofolate reductase gene in a class I integron. The source of the strain remains unresolved. Representative isolates were obtained from various specimens from hospitals and general practice throughout the county, with no evidence of patient-to-patient transmission.",

author = "N. Norskov-Lauritsen and Dorthe Sandvang and J. Hedegaard and V. Fussing and K.K. Mortensen and H.U. Sperling-Petersen and H.C. Schonheyder",

year = "2001",

language = "English",

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TY - JOUR

T1 - Clonal origin of aminoglycoside-resistant Citrobacter freundii isolates in a Danish county

AU - Norskov-Lauritsen, N.

AU - Sandvang, Dorthe

AU - Hedegaard, J.

AU - Fussing, V.

AU - Mortensen, K.K.

AU - Sperling-Petersen, H.U.

AU - Schonheyder, H.C.

PY - 2001

Y1 - 2001

N2 - During 1997, attention was drawn to an increased frequency of aminoglycoside-resistant Citrobacter freundii in a Danish county, when a total of 24 resistant C. freundii isolates was detected. In this study, 15 such isolates were typed by pulsed-field gel electrophoresis, riboprinting and partial sequencing of the gene encoding translation initiation factor 2. Fourteen of the 15 isolates were identical, as evaluated by their antibiograms and by all these typing methods, This epidemic strain harboured the aminoglycoside resistance genes aac(3)-II and ant(3")-I, with the latter located in tandem with a dihydrofolate reductase gene in a class I integron. The source of the strain remains unresolved. Representative isolates were obtained from various specimens from hospitals and general practice throughout the county, with no evidence of patient-to-patient transmission.

AB - During 1997, attention was drawn to an increased frequency of aminoglycoside-resistant Citrobacter freundii in a Danish county, when a total of 24 resistant C. freundii isolates was detected. In this study, 15 such isolates were typed by pulsed-field gel electrophoresis, riboprinting and partial sequencing of the gene encoding translation initiation factor 2. Fourteen of the 15 isolates were identical, as evaluated by their antibiograms and by all these typing methods, This epidemic strain harboured the aminoglycoside resistance genes aac(3)-II and ant(3")-I, with the latter located in tandem with a dihydrofolate reductase gene in a class I integron. The source of the strain remains unresolved. Representative isolates were obtained from various specimens from hospitals and general practice throughout the county, with no evidence of patient-to-patient transmission.

M3 - Journal article

SN - 0022-2615

VL - 50

SP - 636

EP - 641

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

IS - 7

ER -

Clonal origin of aminoglycoside-resistant Citrobacter freundii isolates in a Danish county

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