CLIPPER: An add-on to the Trans-Proteomic Pipeline for the automated analysis of TAILS N-terminomics data

Ulrich Auf Dem Keller*, Christopher M. Overall

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Data analysis in proteomics is complex and with the extra challenges involved in the interpretation of data from N-terminomics experiments, this can be daunting. Therefore, we have devised a rational pipeline of steps to approach N-terminomics data analysis in a statisticallybased and valid manner. We have automated these steps in CLIPPER, an add-on to the Trans-Proteomic Pipeline (TPP). Applying CLIPPER to the analysis of N-terminomics data generated by terminal amine isotopic labeling of substrates (TAILS) enables high confidence peptide to protein assignment, protein N-terminal characterization and annotation, and for protease analysis readily allows protease substrate discovery with high confidence.

Original languageEnglish
JournalBiological Chemistry
Volume393
Issue number12
Pages (from-to)1477-1483
ISSN1431-6730
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Degradomics
  • N-terminomics
  • Protease
  • Proteomics
  • Terminal amine isotopic labeling of substrates (TAILS)
  • Trans-Proteomic Pipeline (TPP)

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